安徽医科大学学报Issue(5)：590-594,5.
氯化锂介导经典Wnt/β-catenin信号通路在大鼠脂肪干细胞增殖和成骨中的作用
Effect of Lithium chloride mediated canonical Wnt/β-catenin signaling on proliferation and osteogenic differentiation of rat adiposed-derived stem cells
摘要
Abstract
Objective To investigate the effect of different concentrations of Lithium chloride on proliferation and osteogenic differentiation of rat adiposed-derived stem cell(ADSCs) in vitro culture and its possible mechanism. Methods ① The ADSCs were harvested from the fat pad in the groin of 3-week-old rats, then maintained in Dul-becco’s modified Eagle’s medium supplemented with 10% fetal bovine serum after digesting with 0 . 1% collagenase type Ⅰ. ADSCs were exposed to Lithium chloride at 0,2. 5,5,10,20,40 mmol/L for 24,48 and 72 hours. The effect of Lithium chloride on cells proliferation was determined by MTT assay.② Alkaline phosphatase( AKP) ac-tivity in cells was detected after ADSCs cultured for 7 days in osteogenic differentiation medium in 2 . 5 ,5 ,10 ,20 ,40 mmol/L or with no Lithium chloride. ③ The ADSCs were treated with different concentrations of Lithium chloride for 7 days after treating with osteogenic differentiation medium for 3 days, and the expressions ofβ-catenin, glyco-gen synthase kinase-3β, AKP were detected by using RT-PCR method. Results In vitro, low doses of Lithium chloride (2. 5,5 and 10 mmol/L) sitimulated ADSCs proliferation,whereas high doses caused a inhibition of prolif-eration . 5 and 10 mmol/L Lithium chloride induce AKP activity in ADSCs which were induced the differentiation to-wards osteolasts,however 40 mmol/L significantly inhibited AKP activity. Meanwhile,Lithium chloride upregulated the expression ofβ-catenin and AKP. Conclusion In vitro,Lithium chloride has a dual effect on ADSCs prolifera-tion, and it improves AKP activity as well as promoting osteogenic differentiation in a dose-dependent manner. 5 mmol/L Lithium chloride can be used as the optimum concentration for stimulating cell proliferation and promoting osteogenic differentiation in rat ADSCs.关键词
Wnt信号通路/脂肪干细胞/氯化锂/增殖/成骨分化Key words
Wnt/β-catenin signaling pathway/adiposed-derived stem cell/Lithium chloride/proliferation/osteo-genic differentiation分类
医药卫生引用本文复制引用
赵璇,徐燕,郑桂婷,沈继龙..氯化锂介导经典Wnt/β-catenin信号通路在大鼠脂肪干细胞增殖和成骨中的作用[J].安徽医科大学学报,2014,(5):590-594,5.基金项目
安徽省自然科学基金(编号:1308085MH130) （编号:1308085MH130）
