安徽医科大学学报Issue(5)：603-605,606,4.
外源性Leptin减轻大鼠肺缺血再灌注损伤及其保护作用机制
Effects and mechanisms of exogenous Leptin alleviating rat lung ischemia-reperfusion injury
摘要
Abstract
Objective Rats serum inflammatory factors and enzyme activities of lung tissue were measured to inves-tigate the protective role and mechanisms of exogenous Leptin alleviating rat lung ischemia-reperfusion injury. Methods Rats lung ischemia-reperfusion injury models were established and one group was treated with exogenous Leptin. Plasma concentrations of C-reactive protein ( CRP) , tumor necrosis factor-α ( TNF-α) and nuclear factor-κB ( NF-κB) were measured by ELISA. The activity of malondialdehyde ( MDA) , myeloperoxidase ( MPO) , su-peroxide dismutase ( SOD) of lung homogenates were measured using colorimetric assay, and the morphological changes were observed under a microscope. Results In ischemia-reperfusion group, CRP, TNF-α, NF-κB con-centration were significantly higher than that of the sham group, and the concentration of exogenous Leptin group was significantly decreased compared with the value of ischemia-reperfusion group ( P<0.05 ) . The lung tissue enzymatic indicators examination also showed that the MPO, MDA levels of exogenous Leptin pretreatment had sig-nificantly decreased ( P<0.05 ) compared with ischemia-reperfusion group ( P<0.05 ) . Histological study showed relatively mild inflammation, edema and bleeding in exogenous Leptin treatment group with alveolar structure intact compared with ischemia-reperfusion injury group. Conclusion Lung ischemia-reperfusion injury was significantly alleviated after application of exogenous Leptin. The exogenous Leptin may play a protective role in lung ischemia-reperfusion injury through reducing lipid peroxidation and inflammatory cell infiltration.关键词
瘦素/肺损伤/肺保护/缺血再灌注Key words
Leptin/lung injury/lung protection/ischemia-reperfusion分类
医药卫生引用本文复制引用
龚文辉,张成鑫,华天凤,汪裕琪,黄大可,赵强..外源性Leptin减轻大鼠肺缺血再灌注损伤及其保护作用机制[J].安徽医科大学学报,2014,(5):603-605,606,4.基金项目
安徽省教育厅高校省级自然科学研究项目(编号:KJ2012Z161) （编号:KJ2012Z161）
