安徽医科大学学报Issue(5)：606-609,4.
DNMT3 A和Hyp在ISO诱导大鼠心肌纤维化中的表达及相关性研究
Expression and correlation of DNMT3 A and Hyp of isoprenaline induced myocardial fibrosis in rats
摘要
Abstract
Objective To investigate the expression and correlation of DNA methyltransferase 3A( DNMT3A) and Hyp of isoprenaline( ISO) induced myocardial fibrosis in rats. Methods 40 male SD rats were randomly divided into normal control group and model group. The model group was treated subcutaneously with 5 mg/( kg·d) iso-prenaline daily for 7 days, but the normal control group was given the same amount of normal saline at the same time. Rats were executed to obtain blood samples and myocardial tissue. And HW/BW, LVW/BW were tested. ELISA was used to detect the content of type Ⅰ collagen and type Ⅲ collagen in serum. The degree of myocardial fibrosis was observed with HE staining and Masson staining method. The expression of DNMT3A and α-SMA was tested by Western blot. The content of hydroxproline in myocardial tissue was measured by UV spectrophotometry. Results HW/BW and LVW/BW increased significantly in the model group compared with the normal control group. The content of type Ⅰ collagen and type Ⅲ collagen in serum increased significantly in the model group compared with the normal control group. HE and Masson staining showed myocardial tissue in model group ap-peared obviously collagen fiber hyperplasia. The expression of DNMT3A andα-SMA in the model group was higher than the normal control group. UV spectrophotometric detection showed the content of Hyp in myocardial tissue in the model group increased obviously. The expression of DNMT3A andα-SMA in myocardial fibrosis tissue was pos-itively correlated(r=0.675, P<0.05). Conclusion DNMT3A upregulation plays an important role in the myo-cardial fibrosis, and Hyp may increase a certain correlation.关键词
心肌纤维化/DNMT3A/Hyp/胶原Key words
myocardial fibrosis/DNMT3 A/Hyp/collagen分类
医药卫生引用本文复制引用
汪裕琪,石开虎,吴君旭,徐盛松,陶辉,宣海洋,曹炜,沙纪名,占红英..DNMT3 A和Hyp在ISO诱导大鼠心肌纤维化中的表达及相关性研究[J].安徽医科大学学报,2014,(5):606-609,4.基金项目
安徽省自然科学基金项目(编号:1308085MH117) （编号:1308085MH117）
安徽省高等学样省级自然科学研究重点项目(编号:KJ2011A175) （编号:KJ2011A175）
