安徽医科大学学报Issue(5)：662-664,3.
耐万古霉素肠球菌耐药表型检测和基因型分析
Detection of phenotype and analysis of genotype in vancomycin-resistant Enterococci
摘要
Abstract
Objective To research the resistant characteristics, genotype and prevalence of vancomycin-resistant Enterococci( VRE) . Methods K-B disc diffusion method was used to determine the susceptibility, minimum inhibi-tory concentration ( MIC) for vancomycin of VRE was detected by E-test method;VRE was then subjected to PCR for resistance related genes;6 strains sequencing results of PCR product were contrastively analyzed the amino acid sequence by BLAST. Results 6 VREFm strains were found from 193 strains enterococci;6 VREFm strains were completely resistant to high unit gentamicin, ampicillin, ciprofloxacin, teicoplanin,but were sensitive to linezolid and macrodantin,MIC for vancomycin was more than 256 mg/L;Genotypes were vanA;Amino acids in vanA gene have changed with Asn83→Asp( AAC→GAC) . Conclusion VRE in our hospital is mostly multi-drug resistant , which bringS difficulty in clinical treatment for its infection. So the hospital should strengthen the prevention and monitoring, to prevent the spread of VRE strains in the hospital and popular.关键词
万古霉素/耐药性/肠球菌/表型/基因型Key words
vancomycin/drug resistance/enterococci/phenotype/genotype分类
医药卫生引用本文复制引用
张侠家,沈继录,贾伟华..耐万古霉素肠球菌耐药表型检测和基因型分析[J].安徽医科大学学报,2014,(5):662-664,3.基金项目
国家自然科学基金(编号:81171618) （编号:81171618）
