安徽医科大学学报Issue(6)：701-705,5.
基质金属蛋白酶1及其抑制剂在白藜芦醇治疗佐剂性关节炎中的表达
Effect of MMP-1,TIMP-1 of resveratrol on rats with adjuvant arthritis
摘要
Abstract
Objective To investigate the expression of matrix metalloproteinases 1 ( MMP-1 ) and tissue matrix metalloproteinases inhibitor-1 ( TIMP-1 ) of synovial tissues and articular cartilage in rats with adjuvant arthritis (AA),to explore whether there is a relation between pathogenesis of AA and expression of MMP-1 and TIMP-1. Methods 60 SD rats were randomly divided into 2 groups:normal control group ( n=10 ) and model group ( n=50). The model group was injected freund's complete adjuvant induced arthritis. When the right paw was induced af-ter 12 days and secondary immune-inflammatory feet were swelled,the rats were randomly divided into 4 groups, and each group had 10 rats:model,resveratrol low dose group(5 mg/kg) , resveratrol high dose group(15 mg/kg) , positive control tripterygium wilfordii glycoside ( TWG, 100 mg/kg ) , qd × 18 day;after inducing inflammatory paw, the expressions of MMP-1 and TIMP-1 in synovial tissue and articular cartilage were detected by immunohis-toehemistry. MMP-1 and TIMP-1 protein content in synovial tissue was detected by Western blot. Results Res-veratrol could inhibit model group rats pathological damage, compared with the AA model group, synovial tissue ex-pression of MMP-1 was significantly higher in resveratrol-treated groups ( P<0 . 05 , P<0. 01 ) , the expression of TIMP-1 was also higher ( P<0. 05 ) . There was a correlation in the role of the anti-inflammation. Conclusion Resveratrol inhibits the inflammation of AA rats and prevents the pathological changes of the joint degeneration, its mechanism is related to regulation of matrix metalloproteinases in synovial tissue of rats and the expression of articu-lar cartilage.关键词
佐剂性关节炎/白藜芦醇/基质金属蛋白酶1/基质金属蛋白酶抑制剂-1/免疫印迹/大鼠Key words
adjuvant arthritis/resveratrol/matrix metalloproteinases 1/matrix metalloproteinases inhibitor-1/Western blot/rats分类
医药卫生引用本文复制引用
安梅,孙和炎,卢锦森,马中飞,纵何香,叶桂萍,郭利梅,何舒宁,陈晓宇..基质金属蛋白酶1及其抑制剂在白藜芦醇治疗佐剂性关节炎中的表达[J].安徽医科大学学报,2014,(6):701-705,5.基金项目
国家自然科学基金(编号:81373421) （编号:81373421）
国家级大学生创新创业训练计划项目(编号:201310366007) （编号:201310366007）
安徽省自然科学基金(编号:1208085MC53) （编号:1208085MC53）
