安徽医科大学学报Issue(7)：863-866,867,5.
TRAIL通过内质网应激对肝星状细胞凋亡的调控及部分机制的研究
Effects of TRAIL on the hepatic stellate cells apoptosis in hepatic stellate cells and relevant mechanism
摘要
Abstract
Objective To detect the effect of ER stress and TRAIL in rat hepatic stellate cell lines ( HSC-T6 ) and to explore the possible apoptosis pathway. Methods Hepatic stellate cell ( HSC) apoptosis was measured by Ho-echst staining apoptosis detection kit and flow cytometric analysis. CHOP,GRP78,C-Caspase-12 and-3 protein ex-pression were analyzed by Western blot;Bax mRNA expression was evaluated by RT-PCR. Results It showed that PDGF-induced HSC as control. The increased of HSC apoptosis was measured by Hoechst 33258 and flow cytome-try. In addition, TRAIL increased the expression of Bax and C-Caspase-3 in activated HSC. TRAIL also increased C-Caspase-12,CHOP and GRP78 in activated HSC. These results suggested that TRAIL induced apoptosis through ERS-mediated pathways in HSC. Conclusion These results demonstrate that TRAIL induces apoptosis through ERS-mediated pathways in HSC.关键词
肿瘤坏死因子相关凋亡诱导配体/肝星状细胞/内质网应激/凋亡/Caspase-12Key words
TNF-related apoptosis inducing-ligand/hepatic stellate cell/apoptosis/endoplasmic reticulum stress/Caspase-12分类
医药卫生引用本文复制引用
李晓慧,李俊,黄艳,黄成,王雅蕊,王欢,孙仕伟,黄蒙蒙..TRAIL通过内质网应激对肝星状细胞凋亡的调控及部分机制的研究[J].安徽医科大学学报,2014,(7):863-866,867,5.基金项目
国家自然科学基金(编号:81072686、81273526、81102493) （编号:81072686、81273526、81102493）
高等学校博士学科点专项科研基金(编号:20103420120001) （编号:20103420120001）
安徽医科大学2013年国家级大学生创新创业训练计划(编号:201310366030) （编号:201310366030）
