安徽医科大学学报Issue(7)：883-886,4.
舒芬太尼后处理对大鼠局灶性脑缺血再灌注损伤的保护作用
Sufentanil postconditioning induces protection against focal cerebral ischemia-reperfusion injury in rats
摘要
Abstract
Objective To investigate whether sufentanil postconditioning induces protection aganist focal cerebral ischemia-reperfusion injury in rats. Methods Seventy healthy adult male SD rats weighing 250~300 g were ran-domly divided into five groups. All rats in IR group,SP1 group,SP2 group and SP3 group were subjected to the right middle cerebral artery occlusion( MCAO) for 90 min and 24 h reperfusion. At 5 min before reperfusion, the rats were intravenously injected sufentanil 0. 3 μg/kg(SP1 group),1 μg/kg (SP2 group), 3 μg/kg (SP3 group) or normal saline ( sham group and IR group ) . The neurological deficit scores ( NDS ) were evaluated at 24 h after reperfusion,then all the animals were executed. Six rats in each group were executed to evaluate the infarct volume of the brain. The remaining rats in each group were executed to detect the apoptosis index. Infarct volume,as a percentage of volume at normal cerebral hemisphere,was determined by 2,3,5-triph-enyltetrazolium ( TTC) stai-ning. The neuronal apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay( TUNEL) . Results At 24 h after reperfusion,the neurological deficit scores and the apoptosis index in the SP2 group and SP3 group were significantly lower than those in the IR group(P<0. 05),the infarct volume in the SP2 group and SP3 group was significantly smaller than that in the IR group(P<0. 05). There were no sig-nificant differences in the neurological deficit scores,apoptosis index and infarct volume between groups of SP1 and IR. Conclusion Sufentanil postconditioning at a dosage of 1 μg/kg can provide neuroprotection against focal is-chemia-reperfusion injury in rats.关键词
舒芬太尼/后处理/脑缺血再灌注损伤Key words
sufentanil/postconditioning/cerebral ischemia-reperfusion分类
医药卫生引用本文复制引用
程岑,顾尔伟,鲁显福,刘训芹,张雷,陈菁菁..舒芬太尼后处理对大鼠局灶性脑缺血再灌注损伤的保护作用[J].安徽医科大学学报,2014,(7):883-886,4.基金项目
国家自然科学基金(编号:81341014) （编号:81341014）
