安徽医科大学学报Issue(7)：891-895,896,6.
重组人内抑素抑制创面瘢痕增生的实验研究
Study of the inhibitory effects of recombinant human endostatin on hypertrophic scars
摘要
Abstract
Objective To investigate the therapeutic effects of recombinant human endostatin ( rhEndostatin ) on hypertrophic scar ( HS ) and explore the possible mechanism of action involved. Methods A rabbit ear model with hypertrophic scars was established. Recombinant human endostatin injections were applied to the scars of the experimental group topically in different concentrations (1. 25, 2. 5, 5 g/L). The changes of macroscopic charac-teristics of scars were examined. Light microscopy and transmission electron microscopy were used for histomorpho-metric analysis. The levels of collagens Ⅰ and Ⅲ were detected by immunohistochemistry. Results Compared with physiological saline group and model group, group treated with rhEndostatin 2. 5 g/L and 5. 0 g/L showed sig-nificant reduction in height, volume and hardness, it resulted in a statistically significant reduction in the scar ele-vation index, the microvessel density and the expression of type Ⅰ and Ⅲ collagens (P<0. 05). An observation under transmission electron microscopy revealed that some fibroblasts nucleus ruptured and disintegrated and apop-totic body were observed in rhEndostatin 5 g/L group, but seldom found in triamcinolone group. Conclusion The findings of this study suggest that rhEndostatin has a markedly inhibitive effect on the formation of hypertrophic scar. It suppresses angiogenesis, and subsequently promotes HSFs apoptosis and reduces the overproduction and excessive deposition of collagen may account for this result.关键词
重组人内抑素/增生性瘢痕Key words
recombinant human endostatin/hypertrophic scar分类
医药卫生引用本文复制引用
余建,张晓明,黄学应,邓雪飞,李小静,焦轶..重组人内抑素抑制创面瘢痕增生的实验研究[J].安徽医科大学学报,2014,(7):891-895,896,6.基金项目
国家自然科学基金面上项目(编号:81171819、81272107) （编号:81171819、81272107）
安徽高校省级自然科学研究项目(编号:KJ2012Z166) （编号:KJ2012Z166）
