安徽医科大学学报Issue(7)：935-940,6.
TACI-Ig对佐剂性关节炎大鼠T细胞反应的调节作用
Immunomodulatory effects of TACI-Ig on T cell responses in rats with adjuvant-induced artiritis
摘要
Abstract
Objective To investigate the immunoregulatory effects of TACI-Ig, a recombinant fusion protein, on T cell responses in an established adjuvant-induced arthritis ( AA) rat model. Methods Rats with experimental ar-thritis were randomly separated into different groups and then treated with TACI-Ig (0. 7, 2. 1, 6. 3 mg/kg), rhT-NFR-Fc (2. 8 mg/kg), MTX (0. 5 mg/kg) or IgG-Fc (6. 3 mg/kg), from d16 to d34 after immunization. Arthri-tis was evaluated by radiographic examination. Levels of IL-17 were assessed by ELISA. The proportion of total, activated, memory and unactivated CD4 + T cells in peripheral blood and spleens were analysed by FACS. TACI ( transmembrane activator and calciummodulator and cyclophilin ligand interactor) , BCMA ( B-cell maturation anti-gen), and BAFF receptor (BAFF-R) expression were analysed by immunohistochemistry. Results TACI-Ig (6. 3 mg/kg) treatment significantly reduced the severity of established arthritis using the methods of radiographic exami-nation. TACI-Ig (0. 7, 2. 1, 6. 3 mg/kg) treatment decreased IL-17 levels both in serum and synovium homoge-nate in AA rats. TACI-Ig (0. 7, 2. 1, 6. 3 mg/kg) increased the proportion of total, activated, memory and unac-tivated CD4 + T cells in peripheral blood and spleens. TACI-Ig (0. 7, 2. 1, 6. 3 mg/kg) administration downregu-lated TACI and BCMA expression and upregulated BAFF-R expression in synovium tissues of AA rats. Conclusion Data presented here demonstrate that administration of TACI-Ig regulates T cell responses and improves joints damage in rats with AA, which may be related to its immnoregulatory effects on expression of BAFF-R, TACI and BCMA in synovium.关键词
TACI-Ig/佐剂性关节炎/类风湿关节炎/T细胞/受体Key words
TACI-Ig/adjuvant-induced artiritis/rheumatoid arthritis/T cell/receptor分类
医药卫生引用本文复制引用
常艳,秦琼,吴育晶,贾晓益,孙晓静,徐澍,魏伟..TACI-Ig对佐剂性关节炎大鼠T细胞反应的调节作用[J].安徽医科大学学报,2014,(7):935-940,6.基金项目
国家自然科学基金(编号:31200675、81173075) （编号:31200675、81173075）
安徽省自然科学基金(编号:1208085QH158) （编号:1208085QH158）
安徽医科大学博士科研资助基金(编号:XJ201215) （编号:XJ201215）
