安徽医科大学学报Issue(7)：984-987,4.
脑胶质瘤组织中EDNRB基因甲基化状态及其蛋白表达的意义
Methylation of EDNRB gene and protein expression in glioma
摘要
Abstract
Abstarct Objective To explore the relation between methylation of EDNRB gene and its protein expression in hu-man brain glial tissues. Methods By using nest PCR, bisulfite PCR and immunohistochemistry staining, we in-vestigated the methylation status at EDNRB gene promoter, EDNRB protein expression and their association with patient clinical history. Results In this study, we found EDNRB gene promoter methylation among 38% of total glioma samples(19/50), with 26. 5% among low grade glioma while 62. 5% among high grade gliomas, which was statistically significant. Within the samples with EDNRB gene methylation events detected, 36. 8% of these sam-ples showed protein expression detected by IHC while 71% samples without EDNRB gene methylation were IHC positive. Conclusion Our study supports the EDNRB gene promoter methylation contributes to gliomagenesis. Mo-reover, the methylation status correlates with grade of glioma but not with patient age, gender, tumor subtype. The methylation status is negative related to protein expression in gliomas.关键词
脑胶质瘤/内皮素受体B/甲基化/蛋白表达Key words
glioma/EDNRB/methylation/protein expression分类
医药卫生引用本文复制引用
成金民,朱立新,王斌,邓鹏程,徐培坤..脑胶质瘤组织中EDNRB基因甲基化状态及其蛋白表达的意义[J].安徽医科大学学报,2014,(7):984-987,4.基金项目
安徽省卫生厅基金(编号:2010B013) （编号:2010B013）
