安徽医科大学学报Issue(9)：1242-1245,4.
水飞蓟素抑制大鼠酒精性脂肪肝库普弗细胞NF-κB活性的研究
Protective effect of silymarin on alcoholic fatty liver in rats possibly via impairing NF-κB activation
摘要
Abstract
Objective To explore the inhibition of NF-κB activation by silymarin in kupffer cells( KCs) in alcohol-ic fatty liver. Methods All rats were randomly divided into normal control group(n=9),model group(n=12) and silymarin groups(100 mg/kg, 200 mg/kg; n=12, respectively). Rat model of alcoholic fatty livers were in-duced by intragastric infusion of ethanol for eight weeks. Histopathological changes of liver were assessed by hema-toxylin and eosin ( HE) staining. Liver KCs were isolated by combined enzymatic digestion and isopyknic gradient centrifugation with nycodenz. The IL-6 and ICAM-1 contents of culture supernatants of KCs were detected by ELISA. NF-κB P65 mRNA expression of KCs was determined by RT-PCR. Results Compared with normal group, the liver cells of model group showed obvious steatosis and significant swelling ( P<0.01 ) . Compared with model group, the degree of pathological changes in liver tissue in silymarin groups was significantly reduced (P<0.01). IL-6 levels in low and high dose of silymarin groups were significantly lower than those of model group ( P<0.01 ) . ICAM-1 levels in low and high dose of silymarin groups were also lower than those of model group(P<0.05, P<0.01 ) . NF-κB P65 mRNA expression in low and high dose of silymarin groups was significantly lower than those of model group(P<0.01). Conclusion Silymarin inhibits NF-κB activation and its gene products, and it may hin-der the development of alcoholic fatty liver.关键词
核转录因子κB/水飞蓟素/脂肪肝/酒精性/细胞间黏附分子-1/白介素-6Key words
nuclear factor-κB/silymarin/fatty liver/alcoholic/intercellular adhesion molecule-1/interleukin-6分类
医药卫生引用本文复制引用
高学梅,洪汝涛,李俊,胡奇珍..水飞蓟素抑制大鼠酒精性脂肪肝库普弗细胞NF-κB活性的研究[J].安徽医科大学学报,2014,(9):1242-1245,4.基金项目
安徽省年度科研计划项目(编号:10020503084) （编号:10020503084）
