安徽医科大学学报Issue(9)：1253-1257,5.
三氯乙烯致敏小鼠Tc1/Tc2细胞失衡研究
The study on imbalance of Tc1/Tc2 cells in trichloroethylene sensitized mice
摘要
Abstract
Objective To detect the alterations of Tc1 and Tc2 cells in trichloroethylene ( TCE ) sensitized mice and to shed light on the possible role of Tc1/Tc2 imbalance on occupational medicamentosa-like dermatitis induced by trichloroethylene ( DMLT) . Methods Female BALB/c mice, randomly separated into blank control, solvent control and TCE group, were sacrificed on 24, 48, 72 h and 7 d after last challenge. Sensitized or non-sensitized mice were identified by cutaneous swelling and histological appearance before sacrifice. Spleens were taken to pre-pare splenocyte suspensions quantifying Tc1 and Tc2 by flow cytometry. Tc lineage-specific transcription factors, T-bet and GATA-3, were evaluated by real-time fluorescence quantitative PCR. Results Compared with non-sensi-tized group, the percentages of Tc1 cells in sensitized mice were significantly higher at four consecutive timepoints (P<0.01), which peaked at 72 h. Tc2 cell levels in sensitized mice were markedly elevated at 24 and 48 h with reaching the summit at 72 h ( P<0.01 ) , whereas the level at 7 d showed no significant difference with non-sensi-tized mice ( P>0.05 ) . Tc1/Tc2 ratios in sensitized group were significantly higher at 48 , 72 h and 7 d compared with non-sensitized group. The levels of T-bet and GATA-3 mRNA in sensitized mice were remarkably increased than corresponding non-sensitized mice at 48 , 72 h and 7 d ( P<0.05 , P<0.01 ) . Conclusion The imbalance of Tc1/Tc2 cells may play a key role in the immunological damage in TCE sensitized mice.关键词
三氯乙烯/细胞毒性T细胞/BALB/c小鼠Key words
trichloroethylene/cytotoxic T cell/BALB/c mice分类
医药卫生引用本文复制引用
张澄,王峰,冷静,査晚生,张家祥,李树龙,王慧,沈彤,朱启星..三氯乙烯致敏小鼠Tc1/Tc2细胞失衡研究[J].安徽医科大学学报,2014,(9):1253-1257,5.基金项目
国家自然科学基金(编号:81371730) （编号:81371730）
高等学校博士学科点专项科研基金(编号:20133420110001) （编号:20133420110001）
