安徽医科大学学报Issue(9)：1274-1278,5.
黄芪甲苷对高糖诱导人肾小球系膜细胞损伤的保护作用及其机制
Protective effects of As-IV on high glucose induced human mesangial cell injury and its mechanisms
摘要
Abstract
Objective To investigate the protective effects of astragaloside IV( As-Ⅳ) on high glucose induced hu-man mesangial cell ( HMC) injury in vitro and its underlying mechanisms. Methods HMC was divided into eight groups and treated as follows: normal group ( NG ) , mannitol ( MA ) , high glucose group ( HG ) , tempol ( 100μmol/L), SB431542 (10 μmol/L), As-IV (25, 50, 100 μmol/L). After treatment for 48 hours,the proliferation was analyzed by MTT assay. The reactive oxygen species (ROS) production was measured by 2, 7-dichlorodihydro fluorescin diacetate ( DCFH-DA) . The level of Col Ⅳ in the supernatant was detected by ELISA. The expressions of transforming growth factor-β1 (TGF-β1),p-Smad2/3,NADPH oxidase 4 (NOX4) and transient receptor poten-tial cation channel 6 (TRPC6) were analyzed by using Western blot. Results Compared with HG group,As-IV, Tempol, SB431542 treatment significantly inhibited HMC proliferation and overproduction of Col Ⅳ induced by high glucose. As-IV, Tempol and SB431542 treatment also decreased expression of TGF-β1, p-Smad2/3, and NOX4 , but significantly increased expression of TRPC6 in HG-cultured HMC ( P<0.05 or P<0.01 ) . Conclu-sion As-IV has protective effects on high glucose induced HMC injury due to inhibit HMC proliferation, enhance anti-oxidant activity, suppress the expression of TGF-β1, p-Smad2/3, and NOX4 and up-regulation of TRPC6 ex-pression.关键词
肾小球系膜细胞/糖尿病肾病/黄芪甲苷/TGF-β1/TRPC6Key words
human glomerular mesangial cell/diabetic nephropathy/astragaloside IV/TGF-β1/TRPC6分类
医药卫生引用本文复制引用
熊莉,李维组,孙立,李贵平,李卫平..黄芪甲苷对高糖诱导人肾小球系膜细胞损伤的保护作用及其机制[J].安徽医科大学学报,2014,(9):1274-1278,5.基金项目
国家自然科学基金(编号:81173624) （编号:81173624）
安徽省自然科学基金(编号:11040606M201) （编号:11040606M201）
安徽省国际合作项目(编号:1230603007) （编号:1230603007）
安徽省教育厅自然科学基金(编号:KJ2012A192) （编号:KJ2012A192）
