安徽医科大学学报Issue(9)：1283-1286,4.
长座虫草子实体和菌丝体中碱基和核苷类物质的HPLC分析
Determination of thirteen nucleosides and nucleobases in the fruiting body and mycelium of Ophiocordyceps longissima by HPLC
摘要
Abstract
An HPLC method was established for simultaneous determination of 13 nucleosides and nucleobases in Ophiocordyceps longissima. The separation was performed on a TSK gel ODS-100V column (250 mmí4.6 mm,5μm) using gradient elution with an aqueous potassium dihydrogen phosphate-methanol solution at 260 nm. The flow rate was 1 . 0 ml/min and column temperature was 20℃. Thirteen nucleosides and nucleobases were analyzed with-in 50 min. The calibration curves showed good linear relationship (r≥0. 999 0, n=8) and the method exhibited excellent accuracy with a recovery of 98. 4 to 107. 5% (RSD<2. 0%,n=5). Higher content of cytosine and hy-poxanthine were detected in the fruiting body. The contents of adenosine, uracil, guanosine were relatively rich in mycelium of Ophiocordyceps longissima. Uridine was abundant both in fruiting body and mycelium of Ophiocordyceps longissima.关键词
长座虫草/高效液相色谱/核苷/碱基/提取/含量测定Key words
Ophiocordyceps longissima/HPLC/nucleosides/nuclebases/extract/determination分类
医药卫生引用本文复制引用
张勋,程文明,李春如,吴昆,陆维丽,李俊..长座虫草子实体和菌丝体中碱基和核苷类物质的HPLC分析[J].安徽医科大学学报,2014,(9):1283-1286,4.基金项目
国家自然科学基金(编号:30570004) （编号:30570004）
天然药物活性组分与功效国家重点实验室资助项目(编号:SKLNMKF201416) （编号:SKLNMKF201416）
安徽省自然科学基金(编号:090413105) （编号:090413105）
