安徽医科大学学报Issue(9)：1320-1324,5.
多途径标本检测aNSCLC患者EGFR基因突变
Detection of EGFR mutation with multiple pathways specimens in advanced non-small cell lung cancer patients
摘要
Abstract
Objective To detect epidermal growth factor receptor ( EGFR) mutations with multiple pathways speci-mens in advanced non-small cell lung cancer ( aNSCLC) patients,and study the relationship between EGFR muta-tion and specimen source,clinical features,pathological features,stage, the value of serum serum carcinoembryonic antigen (CEA). Methods Multiple pathways specimens like tumor tissue,cytology and plasma in aNSCLC pa-tients were collected. QIAGEN’s new QIAamp Circulating Nucleic Acid Kit was used to extract cfDNA in plasma, scorpion probe amplification refractory mutation system ( sARMS) was used for EGFR mutation test. Results The EGFR mutation rate of 103 cases in patients with NSCLC used tissue or cytology specimens was 48.5%(50/103). There was a significant correlation between EGFR mutation smoking history and histological type ( P<0.05 ) . No correlation with gender,age, physical status score, stage and the value of serum CEA. The mutation rate of metas-tases source group ( 58.6%) was higher than the primary tumor source group ( 35.6%) . Patients with malignant pleural effusions (67.5%) were higher than those without pleural effusion (36.5%) (P<0.05);verses tissue or cytology specimens,the EGFR mutation positive rate in 29 cases of paired plasma samples was 37.9%( 11/29 ) , sensitivity was 43.7%(7/16)and specificity was 69.2%(9/13). Conclusion aNSCLC patients with EGFR gene mutation were usually observed in non-smoking and adenocarcinoma patients. There is no significant difference with gender,serum carcinoembryonic antigen,clinical stage and physical status. The EGFR mutation rate is higher in pa-tients with malignant pleural effusion than patients without. Using QIAamp Circulating Nucleic Acid Kit for extrac-ting cfDNA in plasma to detect EGFR mutation,the sensitivity is acceptable. If cheap, plasma may be a comple-mentary approach for tumor tissue. Due to the existence of intratumoral EGFR mutational heterogeneity, obtaining different parts of the tumor tissue is essential.关键词
非小细胞肺癌/表皮生长因子受体/血浆Key words
NSCLC/EGFR/plasma分类
医药卫生引用本文复制引用
冷再君,徐傲,徐修才,徐飞,伍权,操乐杰..多途径标本检测aNSCLC患者EGFR基因突变[J].安徽医科大学学报,2014,(9):1320-1324,5.基金项目
安徽省卫生厅医学科研课题计划(编号:13ZC001) （编号:13ZC001）
安徽省科技攻关计划项目(编号:1301042216) （编号:1301042216）
