安徽医科大学学报Issue(10)：1382-1386,5.
人通用转录因子GTFIIF2表达与定位
Human induced the expression of GTFIIF2 in BL21 and the localization in cells
摘要
Abstract
Objective To construct recombinant plasmids of GTFIIF2 to detect the expression and the expression and localization of GTIFIIF2 in cell. Methods The primers of GTFIIF2 were designed,GTFIIF2 was amplified by PCR with the template including the full length cDNA fragment of GTFIIF2,to construct pcDNA3. 1-FLAG-GTFIIF2 and pCDGFP-GTFIIF2 vectors,the exprassion and localization of GTFIIF2 were investigated by the method of West-ern blot and immunofluoresent assay. Vector GST-GTFIIF2 was transformed into Eicoli BL21 to observe the expres-sion of fusion protein in the induction of IPTG. Results The protein of GIFIIF2 was expressed efficiently in the nu-cleus of COS7 cells not in the cytoplasm;pcDNA3 . 1-FLAG-GTFIIF2 and pCDGFP-GTFIIF2 plasmid could be effec-tively expressed in HEK293T cells;GST-GTFIIF2 could also be induced in the BL21 strains. Conclusion GTFIIF2 can effectively express in COS7 cells,HEK293T cells and BL21 competent cells. The results provide a better un-derstanding of the basic reasearch of GTFIIF2 functions in cells.关键词
GTFIIF2/基因表达/蛋白定位/BL21Key words
GTFIIF2/gene expression/protein localization/BL21分类
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赵健,耿慧武,乔正,李春雨,李克娟,刘晓颖,范礼斌..人通用转录因子GTFIIF2表达与定位[J].安徽医科大学学报,2014,(10):1382-1386,5.基金项目
国家自然科学基金青年基金(编号:81201368) （编号:81201368）
安徽省自然科学基金项目(编号:11040606M170、11040606M164) （编号:11040606M170、11040606M164）
安徽省教育厅自然科学重点科研项目(编号:KJ2010A187) （编号:KJ2010A187）
