安徽医科大学学报Issue(10)：1387-1391,5.
生长抑素对瘦素诱导肝星状细胞的活化增殖、PTP1 B表达及JAK2-STAT3通路的影响
Effect of somatostatin on leptin-induced activation, proliferation, PTP1 B expression and JAK2-STAT3 signaling of hepatic stellate cells
摘要
Abstract
Objective To investigate the effect of somatostatin on leptin-induced activation, proliferation, PTP1B expression and JAK2-STAT3 signaling of rat hepatic stellate cells ( HSC-T6 ) . Methods HSC-T6 cells were trea-ted with recombinant rat leptin (100 ng/ml) and various concentrations of somatostatin (10 -6,10 -7,10 -8,10 -9, 10 -10 mol/L). After incubation for 24 h, the cell proliferation rates of the groups were determined by CCK-8. Im-munocytochemical staining assay was applied to evaluate phosphorylation of Janus kinase 2 ( JAK2 ) and protein ex-pression of signal transducers and activators of transcription 3 ( STAT3 ) . The mRNA expression of α-SMA and PTP1B was evaluated by RT-PCR. Results With CCK-8, somatostatin could promote leptin-induced proliferation of hepatic stellate cells in a dose-adependent manner ( P<0.05 ) . The expressions of cytoplasmic p-JAK2 and nu-cleic p-STAT3 after somatostatin intervention were significantly lower than that of the leptin control group ( P <0.05). Compared with the group with leptin,α-SMA mRNA of somatostatin group was significantly down-regulated after the treatment ( P<0.05 ) , while PTP1 B mRNA expression was significantly increased ( P<0.05 ) . Conclu-sion Somatostatin up-regulates PTP1B expression, inhibits JAK2-STAT3 signal transduction in HSC and the cell activation and proliferation induced by leptin.关键词
生长抑素/瘦素/肝星状细胞/JAK2-STAT3信号转导/蛋白酪氨酸磷酸酶1BKey words
somatostatin/leptin/hepatic stellate cell/JAK2-STAT3 signal transduction/protein tyrosine phos-phatase 1 B分类
医药卫生引用本文复制引用
李望,张超,李方跃,牛森森..生长抑素对瘦素诱导肝星状细胞的活化增殖、PTP1 B表达及JAK2-STAT3通路的影响[J].安徽医科大学学报,2014,(10):1387-1391,5.基金项目
安徽省教育厅自然科学重点项目(编号:KJ2014A117) （编号:KJ2014A117）
