安徽医科大学学报Issue(10)：1392-1395,4.
促炎因子在实验性重症急性胰腺炎早期对JAK2/STAT3信号通路的影响
Correlation of proinflammatory cytokines and JAK/STAT signaling pathway in early severe acute pancreatitis
摘要
Abstract
Objective To investigate the relationship between proinflammatory cytokines and JAK2/STAT3 signal pathway in early severe acute pancreatitis. Methods The rat model of SAP was reproduced by retrograde infusion of 4% sodium taurocholate into the biliopancreatic duct. Thirty-two male SD rats were randomly assigned into 4 groups (8 each):normal control group, SAP 6 h, 12 h and 18 h groups. The levels of serum amylase, lipase and calcium level were measured dynamically, pathological changes in pancreas was evaluated. The concentrations of TNF-α, IL-1β and IL-18 were determined by ELISA, and the expressions of JAK2 and STAT3 protein and mRNA in pancrease was also determined by Western blot and RT-PCR. Results The SAP module groups serology level, TNF-α, IL-1βand IL-18 were significantly higher than those in NC group. The microscopic pancreas injuries were gradually aggravated with disease progression. The expression of JAK2 and STAT3 gene and protein in experimental groups were significantly increased compared with NC group, and reached the peak in 18h group. Conclusion Se-vere acute pancreatitis proinflammatory cytokines overexpression is damage factor progression of disease, which may activate the JAK2/STAT3 signaling pathway, aggravating the organ injury in early stage of severe acute pancreati-tis.关键词
重症急性胰腺炎/促炎因子/JAK2/STAT3通路/胰腺损伤Key words
severe acute pancreatitis/proinflammatory cytokines/JAK2/STAT3 signaling pathway/pancreas in-jury分类
医药卫生引用本文复制引用
李敏利,许小兵,王彬,金鑫鑫,郭美霞,张晓华..促炎因子在实验性重症急性胰腺炎早期对JAK2/STAT3信号通路的影响[J].安徽医科大学学报,2014,(10):1392-1395,4.基金项目
南京军区总医院科研基金(编号:2012064) （编号:2012064）
