安徽医科大学学报Issue(10)：1396-1399,4.
TGF-β3在小鼠放射性肺纤维化中的作用研究
Effect of TGF-β3 on radiation-induced pulmonary fibrosis of mice
摘要
Abstract
Objective To observe the changes of lungs in mice after 60 Co γ-ray irradiation on whole breast,and to explore the role of TGF-β3 in radiation-induced pulmonary fibrosis in mice. Methods 180 C57BL/6 female mice were randomly divided into control group,irradiation group and TGF-β3 group. The mice in irradiation group and TGF-β3 group were irradiated once by 60 Coγ-ray with does of 20 Gy on whole chest. Then,the mice of both groups above were intraperitoneal injected 0. 9% saline and TGF-β3 (1 μg/kg) 0. 5 ml respectively once a week. Kill the mice at 3,7,14 days and 1,3,6 months after irradiation. To observe pathology of lungs by HE staining and Sirius red staining,and to detect the number of CF in lungs of mice. Results To establish the model of radiation-induced pulmonary fibrosis in mice. 6 months after irradiation,the lungs in TGF-β3 group appeared proliferation of some fi-broblasts and deposition of a small amount collagen. From 14 days to 6 months after irradiation,the number of CF in lungs in irradiation group was significantly higher(P<0.05) than control group. 1 month after irradiation,number of CF in lungs in TGF-β3 group was significantly lower ( P<0.05 ) than irradiation group. Conclusion TGF-β3 obviously reduces the accumulation of CF in lungs of mice after radiation,which may play an important role in dela-ying the occurrence of pulmonary fibrosis.关键词
放射性肺纤维化/循环纤维细胞/TGF-β3Key words
radiation-induced pulmonary fibrosis/circulating fibrocytes/TGF-β3分类
医药卫生引用本文复制引用
汪倩君,徐龙,熊珊珊,肖凤君,杨陟华,潘秀颉,朱茂祥..TGF-β3在小鼠放射性肺纤维化中的作用研究[J].安徽医科大学学报,2014,(10):1396-1399,4.基金项目
国家自然科学基金(编号:81001219) （编号:81001219）
