安徽医科大学学报Issue(10)：1400-1403,1404,5.
miR-107对HepG2细胞增殖和细胞周期的影响
The effects of miR-107 on proliferation and cell cycle of HepG2 cells
摘要
Abstract
Objective To investigate the effects of miR-107 on cell proliferation and cell cycle of HepG2 cells, as well as the expression of CDK 6 protein . Methods Human hepatoma HepG 2 cells were transfected with 50 nmol/L miR-107 mimics or negative control using Lipofectamine 2000. After transfection, the expression level of miR-107 was tested by qRT-PCR. Cell proliferation was tested by methylthiazol tetrazolium ( MTT) assay. Cell cycle was de-tected by flow cytometry. The expression level of CDK6 protein was measured by Western blot. Results Compared with the blank control group, the expression level of miR-107 in miR-107 mimics group was significantly increased (P<0.05). In addition, the proliferation of HepG2 cells was inhibited(P<0.05), G0/G1 phase cells were in-creased ( P<0.05 ) , and the expression level of CDK6 protein was decreased ( P <0.05 ) in miR-107 mimics group. Conclusion These results demonstrate that miR-107 inhibits the proliferation and induces G0/G1 phase ar-rest in HepG2 cells, which may be related to the inhibition of CDK6 expression.关键词
肝癌/miR-107/增殖/细胞周期/CDK6Key words
hepatocellular carcinoma/miR-107/proliferation/cell cycle/CDK6分类
医药卫生引用本文复制引用
贾振亚,曹静,范文洁,徐玥,范璐璐,孙国平..miR-107对HepG2细胞增殖和细胞周期的影响[J].安徽医科大学学报,2014,(10):1400-1403,1404,5.基金项目
国家自然科学基金(编号:81272739、81071986) （编号:81272739、81071986）
