miR-107对HepG2细胞增殖和细胞周期的影响OA北大核心CSTPCD
The effects of miR-107 on proliferation and cell cycle of HepG2 cells
目的探讨miR-107对HepG2细胞增殖和细胞周期的影响,以及对CDK6蛋白表达的调控。方法将50 nmol/L的miR-107 mimics 或阴性对照序列由 Lipofectamine 2000转染入人肝癌细胞株 HepG2中；qRT-PCR 法检测转染后HepG2细胞内miR-107表达水平；四甲基偶氮唑蓝( MTT)法检测细胞增殖能力；流式细胞术分析转染后细胞周期的变化；Western blot法检测CDK6蛋白表达水平。结果转染后24…查看全部>>
Objective To investigate the effects of miR-107 on cell proliferation and cell cycle of HepG2 cells, as well as the expression of CDK 6 protein . Methods Human hepatoma HepG 2 cells were transfected with 50 nmol/L miR-107 mimics or negative control using Lipofectamine 2000. After transfection, the expression level of miR-107 was tested by qRT-PCR. Cell proliferation was tested by methylthiazol tetrazolium ( MTT) assay. Cell cycle was de-tected by flow cytome…查看全部>>
贾振亚;曹静;范文洁;徐玥;范璐璐;孙国平
安徽医科大学第一附属医院肿瘤科，合肥 230022安徽医科大学第一附属医院肿瘤科，合肥 230022安徽医科大学第一附属医院肿瘤科，合肥 230022安徽医科大学第一附属医院肿瘤科，合肥 230022安徽医科大学第一附属医院肿瘤科，合肥 230022安徽医科大学第一附属医院肿瘤科，合肥 230022
医药卫生
肝癌miR-107增殖细胞周期CDK6
hepatocellular carcinomamiR-107proliferationcell cycleCDK6
《安徽医科大学学报》 2014 (10)
肝癌细胞逃避内质网应激介导的凋亡的分子机制及丹皮酚的干预作用
1400-1403,1404,5
国家自然科学基金(编号:81272739、81071986)
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