安徽医科大学学报Issue(1)：9-11,12,4.
p22-phox在P19细胞向心肌细胞分化前后的变化
The changes of the expression of p22-phox before and after P19 cells differentiating into cardiac myocytes
摘要
Abstract
Objective To investigate the change of the expression of p22-phox before and after P19 cells differenti-ating to cardiac myocytes. Methods Appropriate P19 cells were chosen which were cultured in vitro with 0. 9%dimethyl sulfoxide( DMSO) , then the induced cells were collected at different times. Western blot of cardiac tropo-nin I ( cTnI) was used to identify cell differentiation, and detect the level of p22-phox before and after the differen-tiation. Results ① On the 8th day cTnI was detected positive in P19 cells which were cultured with 0. 9% DM-SO, then the expression of cTnI was increased, with statistical significance(P<0. 05).② The expression of p22-phox in differentiated P19 cells was higher than that in undifferentiated P19 cells, with statistical significance( P<0. 05) . Conclusion The expression of p22-phox increases during the differentiation of P19 cells to cardiac myo-cytes, and the level of oxidative stress increases.关键词
P19细胞/心肌细胞/细胞分化/氧化应激/p22-phoxKey words
P19 cells/cardiac myocytes/cell differentiation/oxidative stress/p22-phox分类
医药卫生引用本文复制引用
吴继军,韩卫星,刘超,陈晓蓉,王成,卢挪挪..p22-phox在P19细胞向心肌细胞分化前后的变化[J].安徽医科大学学报,2015,(1):9-11,12,4.基金项目
国家自然科学基金(编号:81070210) （编号:81070210）
