安徽医科大学学报Issue(1)：16-19,4.
NF-κB沉默抑制鼻咽癌5-8 F细胞增殖
NF-κB silencing inhibits the proliferation of nasopharyngeal carcinoma 5-8 F cell
摘要
Abstract
Objective To evaluate the inhibition effects of nuclear factor-κB( NF-κB) silencing in the nasopharyn-geal carcinoma 5-8F cells. Methods Constructing the NF-κB SiRNA expression vector pGenesil-1. 2-NF-κB and emptor pGenesil-1. 2-HK,and then they were transfected into nasopharyngeal carcinoma 5-8F cells. Western blot was used to detect expression of NF-κB p65 protein. MTT and flow cytometry were used to evaluate the proliferation inhibition and the cell cycle arrest in 5-8F cells respectively. Results NF-κB p65 silencing could inhibit the pro-liferation of nasopharyngeal carcinoma 5-8F cells and the inhibition rate was (58. 43 ± 1. 24)%,0% in the control group and (17. 89 ± 4. 13)% in the empty vector control group, and the difference was statistically significant ( P<0. 05). The cell number of S phase was (42. 27 ± 0. 39)% in NF-κB silencing group, (30. 83 ± 1. 36)% in the control group and (34. 88 ± 0. 85)% in the empty vector control group, and the difference was statistically signifi-cant ( P<0. 05 ) . Conclusion The NF-κB p65 knockdown decrease cell proliferation in nasopharyngeal carcino-ma 5-8F cells, maybe due to cell cycle arresting in S phase.关键词
NF-κB/鼻咽癌/siRNAKey words
nuclear factor-κB/nasopharyngeal cancer/siRNA分类
医药卫生引用本文复制引用
潘红杰,范才文,易世江,李璐,刘建翔,王建红,罗琴,向秋..NF-κB沉默抑制鼻咽癌5-8 F细胞增殖[J].安徽医科大学学报,2015,(1):16-19,4.基金项目
国家自然科学基金(编号:81260344) （编号:81260344）
广西研究生教育创新项目(编号:YCSZ2012110) （编号:YCSZ2012110）
广西高等学校优秀人才资助计划项目 （）
广西自然科学基金(编号:2013GXNSFAA019228) （编号:2013GXNSFAA019228）
桂林市科学研究与技术开发计划项目(编号:20130120-19) （编号:20130120-19）
