安徽医科大学学报Issue(1)：45-48,49,5.
抗炎因子TSG-6抑制兔耳瘢痕增生的实验研究
The experimental study of anti-inflammatory cytokine TSG-6 inhibits hypertrophic scar formation in rabbit ears model
摘要
Abstract
Objective To observe the effect of tumor necrosis factorαstimulated gene-6 ( TSG-6 ) on hypertrophic scarring by using a rabbit ear model. Methods TSG-6 and PBS were injected intradermally in the right and left ear wounds, respectively. Collagen I and III expression detected by immunohistochemistry and scar elevation index ( SEI) was used to evaluate the extent of scarring. The expression of inflammatory factors interleukin-1β( IL-1β) , interleukin-6 ( IL-6 ) and tumor necrosis factor-α( TNF-α) was detected by immunohistochemistry and reverse tran-scription polymerase chain reaction. Transmission electron microscope ( TEM) and TUNEL analyses were used to detect fibroblast apoptosis. Results Compared with control scars, TSG-6-treated wounds exhibited decreased in-flammation significantly as evidenced by the lower levels of IL-1β, IL-6 , TNF-α. The apoptosis rate was higher and the SEI and the synthesis of collagens I and III were significantly decreased in the TSG-6-treated scars ( P<0. 05 ) . Conclusion Immediate topical injection of TSG-6 during the wound healing process can reduce the severity of hy-pertrophic scarring in a rabbit model. The anti-cicatrix effect of TSG-6 may result from controlling inflammation, in-ducing fibroblast apoptosis and promoting collagen degradation.关键词
动物模型/增生性瘢痕/兔/炎症/TSG-6Key words
animal model/hypertrophic scar/rabbit/inflammation/TSG-6分类
医药卫生引用本文复制引用
王晖,李小静,陈钊..抗炎因子TSG-6抑制兔耳瘢痕增生的实验研究[J].安徽医科大学学报,2015,(1):45-48,49,5.基金项目
国家自然科学基金(编号:81272107) （编号:81272107）
