安徽医科大学学报Issue(2)：140-143,4.
内质网应激标志性蛋白Caspase-12在四氯化碳诱导大鼠肝纤维化逆转恢复模型中的作用研究
Effect of endoplasmic reticulum stress protein caspase-12 in carbon tetrachloride-induced reversible liver fibrosis in rats
摘要
Abstract
Objective To detect the effect of endoplasmic reticulum stress ( ERS)-related protein Caspase-12 in rat liver fibrosis and spontaneous reversal process. Methods Liver fibrosis model was induced by CCl4 in rats, the protein expressions of Calpain andα-SMA were detected by immunohistochemical staining. The protein expressions of Calpain,Cleaved-Caspase-12 and Cleaved-Caspase-3 in liver tissue were measured by Western blot. Results Immunohistochemistry results showed that compared with the normal group,α-SMA and calpain antigen-positive cells of rat liver tissue in model group were significantly increased, distributing in liver parenchymal area. Com-pared with model group,in recovery model,α-SMA antigen-positive cells decreased. Calpain antigen-positive cells were mainly distributed in the liver lobule central vein, portal area, sinusoidal gap and other parts. Western blot results showed that compared with the control group, expressions of Calpain, Cleaved-Caspase-12 and Cleaved-Caspase-3 were significantly enhanced in the model group. Compared with the model group, expressions of Cal-pain,Cleaved-Caspase-12 and Cleaved-Caspase-3 were decreased in spontaneous recovery after 2, 4 and 8 weeks. Conclusion ERS-related protein Caspase-12 might be related to the progression and regrossion of liver fibrosis.关键词
肝纤维化/内质网应激/凋亡/Calpain/Caspase-12Key words
liver fibrosis/endoplasmic reticulum stress/apoptosis/Calpain/Caspase-12分类
医药卫生引用本文复制引用
黄艳,李晓慧,吴正升,王欢,汪应红,李海迪,孙仕伟,李俊..内质网应激标志性蛋白Caspase-12在四氯化碳诱导大鼠肝纤维化逆转恢复模型中的作用研究[J].安徽医科大学学报,2015,(2):140-143,4.基金项目
国家自然科学基金资助项目(编号:81102493、30873081) （编号:81102493、30873081）
国家教育部博士点新教师基金资助项目(编号:20103420120001) （编号:20103420120001）
安徽医科大学国家级大学生创新创业训练计划项目(编号:201310366030) （编号:201310366030）
