安徽医科大学学报Issue(2)：144-148,149,6.
尿液POU4 F2基因甲基化对膀胱尿路上皮癌早期诊断的意义
Diagnosis of bladder cancer based on urinary levels of POU4 F2 hypermethylation
摘要
Abstract
Objective To identify a panel of novel epigenetic biomarkers with high sensitivity and specificity that can be utilized in detection and diagnosis of bladder cancer using urine sediments. Methods T24 cell lines that had been treated with bisulfite were used to examine the 8 methylated candidates that were previously reported in bladder cancer patients. Methylation levels of the candidate genes were quantified using the urine sediments from 28 bladder cancer patients, 30 healthy volunteers and 10 infected urinary calculi patients by quantitative methylation-specific polymerase chain reaction(qMSP). The four most efficacious and reliable biomarkers were selected after the sensitivity and specificity of each biomarker were further calculated and inspected. Results The sensitivities of PCDH17, POU4F2, TCF21 and ZNF154 in the detection of bladder cancer were 46. 43%, 92. 86%, 39. 29% and 46. 43% respectively;the specificities of these biomarkers were 95. 00%, 97. 50%, 97. 50% and 100. 00% re-spectively. POU4F2 appeared as the best biomarkers among the four, showing a sensitivity of 92. 86% and a speci-ficity of 97 . 50%. Conclusion Bladder cancer can be detected by a biomarker panel by using qMSP depending on the urine samples from patients.关键词
膀胱癌/qMSP/甲基化/诊断Key words
bladder cancer/qMSP/hypermethylation/diagnosis分类
医药卫生引用本文复制引用
王永强,于源,安丹,姜会玲,林友成,吴松,蔡志明..尿液POU4 F2基因甲基化对膀胱尿路上皮癌早期诊断的意义[J].安徽医科大学学报,2015,(2):144-148,149,6.基金项目
国家重点基础研究发展计划(973计划项目)(编号:2014CB745200) （973计划项目）
