安徽医科大学学报Issue(2)：149-153,5.
酒精性脂肪肝中库普弗细胞表型变化的探讨
The study of kupffer cells phenotypic changes in alcoholic fatty liver
摘要
Abstract
Objective Establish the model of alcoholic fatty liver in mice and isolate the biological activity kupffer cells (KCs), in order to study its role in the alcoholic fatty liver disease. Methods C57BL/6 mice were fed with the Lieber-DeCarli diet for 16 days plus one time of acute alcohol lavage,to establish the model of alcoholic fatty liver. Tissue specimens were collected on the sixteenth day after gastric lavage 9 h later. To verify whether the model was established successfully by detecting the level of serum alanine aminotransferase/glutamic oxalacetic transaminase( ALT/AST) and total cholesterol/triglycerides( TG/TC) , the level of TG/TC of liver tissue homoge-nate and liver pathological section HE and oil red staining. KCs were isolated by in situ perfusion, and the cell yield and cell changes were detected by flow cytometry. Cytokine levels of organization and the primary cell were detected by qRT-PCR. Results The model response to liver injury index of ALT/AST was higher than that of con-trol group,meanwhile HE and oil red staining results were consistent with that. So the alcoholic fatty liver model was successfully established in mice. Cell yield of each mouse was about 1. 5 × 106 ~2. 0 × 106 , KCs were double-labeled by murine macrophage cell surface marker F4/80 molecule and white blood cells surface antigen molecules CD45, F4/80 and CD45 double positive cells were selected to flow analysis. Data showed that natural CD68 + was significantly lower in the model, a large number of the infiltration of mononuclear cells were elevated. Florescent real-time quantitative RT-PCR(qRT-PCR) results showed that cytokine,tumor necrosis factor (TNF-α),interleu-kin-6 ( IL-6 ) , monocyte chemoattractant protein ( MCP-1 ) level increased significantly in liver tissue and primary cell. Conclusion The model of alcoholic fatty liver is successfully established and there is a high yield of cells in situ perfusion. The incidence of alcoholic fatty liver may be relative to hepatic macrophages constitute and pheno-typic changes, associated with elevated cytokine mediated infiltration of peripheral mononuclear cells.关键词
酒精性脂肪肝/原位灌流/库普弗细胞/细胞因子Key words
alcoholic fatty liver/in situ perfusion/kupffer cells/cytokines分类
医药卫生引用本文复制引用
程希,胡超杰,李晚霞,黄成,李俊..酒精性脂肪肝中库普弗细胞表型变化的探讨[J].安徽医科大学学报,2015,(2):149-153,5.基金项目
国家自然科学基金(编号:81273526) （编号:81273526）
安徽省教育厅基金项目(编号:KJ2012A156) （编号:KJ2012A156）
安徽省自然科学基金(编号:1308085MH145) （编号:1308085MH145）
安徽医科大学博士科研启动基金(编号:XJ201118) （编号:XJ201118）
