安徽医科大学学报Issue(3)：294-297,4.
西格列汀对2型糖尿病大鼠chemerin水平的影响
The influence of sitaglipin on chemerin levels of type 2 diabetic rats
摘要
Abstract
Objective To investigate the effects of sitagliptin on metabolic parameters and chemerin levels of type 2 diabetic rats, and to explore the relationship between chemerin and type 2 diabetes. Methods A total of 35 male SD rats were randomly divided into normal diet group (NC, n=10) and a high-fat diet group (HF, n=25). Af-ter 12 weeks, high-fat diet rats were given an injection of 35 mg/kg streptozotocin at a time,then the diabetic model rats (n=20) were randomly divided into 2 groups:diabetes control group (T2DM, n=10) and sitagliptin inter-vention group (SP, n=10). The rats'metabolic parameters were measured by ELISA. The chemerin protein expres-sion levels in kidney adipose, liver and muscle tissue were detected by Western blot. Results ① The serum chemerin,FBG, FINS, TCH, TG, LDL, HOMO-IR levels of diabetic model group were increased. HOMO-beta decreased. Chemerin expression levels of diabetes control group in adipose, liver and muscle tissue increased com-pared with NC group;② Sitagliptin could down-regulate the levels of BW, FBG, FINS, TC, TG, LDL, HOMO-IR,serum chemerin and chemerin levels in adipose and liver tissues. On the contrary,HOMO-beta was up-regula-ted;③ The correlation analysis showed that TG, FINS and HOMO-IR were always independently associated with chemerin. In tissues, serum chemerin had positive correlation with chemerin expression levels in adipose. Conclu-sion Sitagliptin can reduce the levels of chemerin in serum and tissues. It also can improve the metabolic indexes remarkably in serum. Chemerin may be a key involving element in the development of diabetes.关键词
2型糖尿病/西格列汀/chemerinKey words
type 2 diabetes/sitagliptin/chemerin分类
医药卫生引用本文复制引用
刘炯炯,王长江,胡红琳,戚仁娟,夏莉,方朝晖..西格列汀对2型糖尿病大鼠chemerin水平的影响[J].安徽医科大学学报,2015,(3):294-297,4.基金项目
安徽省高校省级自然科学研究项目(编号:KJ2013Z126) （编号:KJ2013Z126）
安徽中医学院中医药管理局重点学科-中医内分泌学科开放基金(编号:2011nfmxk004) （编号:2011nfmxk004）
安徽医科大学第一附属医院2001年度国家自然科学基金青年科学基金培养计划(编号:2011KJ11) （编号:2011KJ11）
