安徽医科大学学报Issue(4)：419-422,4.
吉西他滨耐药的胰腺癌SW1990细胞株的建立及相关特性检测
Establishment of the human resistant pancreatic cancer cell lines SW1990/gemcitabine and its characteristics test
摘要
Abstract
Objective To investigate how to induce the resistant cell line SW1990/gemcitabine( GEM) for further clarify the resistant mechanisms of pancreatic cancer , and compare the characteristics between SW1990 and SW1990-GZ. Methods SW1990-GZ was derived from the human pancreatic cancer cell lines SW1990 by exposing the cells to intermittently increasing concentrations of GEM. The IC50 ,resistance index( R) , and growth difference was observed by MTT assay. Cellular intake of SW1990 and SW1990-GZ was detection by HPLC. Results The IC50 of SW1990 and SW1990-GZ were respectively (0. 07 ± 0. 002 1)μg/ml,(87. 50 ± 3. 240 0)μg/ml,R=1 250;sensibility to GEM in SW1990-GZ were more lower than that in SW1990 cell in different concentration s of GEM. Growth rate of SW1990-GZ was slower than that of SW1990 from the growth curve;GEM intake in SW1990-GZ was lower than that in SW1990 in different times. Conclusion Human resistant pancreatic cancer cell lines SW1990/GEM is successfully established,and its resistant characteristics are stable and clear. SW1990-GZ maybe decrease GEM intake by some unclear pathways.关键词
人类胰腺癌细胞株/吉西他滨耐药/建立Key words
human pancreatic cancer cell lines/gemcitabine resistance/establish分类
医药卫生引用本文复制引用
贾一夫,丁飞,余跃,高萌,杨显珠,王均..吉西他滨耐药的胰腺癌SW1990细胞株的建立及相关特性检测[J].安徽医科大学学报,2015,(4):419-422,4.基金项目
安徽省自然科学基金(编号:1208085MH174) （编号:1208085MH174）
