安徽医科大学学报Issue(4)：427-431,5.
胰腺癌细胞与其基质成纤维细胞相互影响的研究
The interaction between pancreatic cancer cells and fibroblasts
摘要
Abstract
Objective To investigate whether the pancreatic cancer cells BXPC-3,SW1990 can activate normal fi-broblasts( NFs) into cancer-associated fibroblasts( CAFs) and the possible mechanism and whether CAFs can moti-vate the migration of the cancer cells. Methods NFs were isolated from mouse pancreas tissues of wild C57,then NFs were cultured with BXPC-3 or SW1990 cells by indirect co-cultures, and detected the content of the specific proteins α-SMA, FAP in fibroblasts by immunofluorescence assay, then detected the content of the 12 different kinds of miRNAs in fibroblasts which were regarded as overexpressed in pancreatic cancer by qRT-PCR, and esti-mated the effect of the different fibroblasts( NFs,CAFs) on cancer cell migration by transwell. Results After coc-ultered with BXPC-3 or SW1990 cells, the α-SMA and FAP level in fibroblasts increased dramatically. Among the selected 12 kinds of miRNAs, after cocultered with BXPC-3 or SW1990 cells,the content of miR-155 in fibroblasts raised significantly. The migration of BXPC-3 ,SW1990 cells increased dramatically in the environment of CAFs compared with the environment of NFs. Conclusion BXPC-3 or SW1990 cells can activate NFs into CAFs, and miRNAs may play an important role in the process,then CAFs can reversely motivate the migration of BXPC-3 or SW1990 cells.关键词
胰腺癌/癌相关成纤维细胞/miRNAs/迁移Key words
pancreatic cancer/cancer-associated fibroblasts/miRNAs/transwell分类
医药卫生引用本文复制引用
苏娇娇,冯慧,姚玮艳,陈熹,张辰宇,王亚雷..胰腺癌细胞与其基质成纤维细胞相互影响的研究[J].安徽医科大学学报,2015,(4):427-431,5.基金项目
国家自然科学基金(编号:81101849/H1617) （编号:81101849/H1617）
