安徽医科大学学报Issue(5)：581-584,4.
TGF-β1和 EGFP 体外电转染兔骨髓间充质干细胞的实验研究
TGF-β1 and EGFP electropration on rabbit bone marrow derived mesenchymal stem cells in vitro
摘要
Abstract
Objective To observe cases of type Ⅱ collagen expression after transforming growth factor-β1 (TGF-β1) infected in rabbit bone mesenchymal stem cells( BMSCs) by electropration. Methods BMSCs from rabbit were isolated and cultured. 14 days after induction, immunohistochemistry and Western blot methods type Ⅱ colla-gen were used to detect the expression of type Ⅱ collagen. Results CD90 was positive and CD31 was negative by flow cytometry;successfully transfected with TGF-β1 to the BMSCs,the strong expression of type Ⅱ collagen in TGF-β1 group cells was shown by Western blot and immunocy-tochemical stain, which was compared with empty plasmid group and the control group,and the difference was statistically significant(P < 0. 05). Conclusion TGF-β1 plasmid by electroporation to rabbit BMSCs can facilitate expression of collagen Ⅱ.关键词
TGF-β1/骨髓间充质干细胞/电转染/Ⅱ型胶原Key words
transforming growth factor beta 1/mesenchymal stem cells/electropration/collagen Ⅱ分类
医药卫生引用本文复制引用
文剑明,王锐英,高燕,胡译文,陶波,周文静..TGF-β1和 EGFP 体外电转染兔骨髓间充质干细胞的实验研究[J].安徽医科大学学报,2015,(5):581-584,4.基金项目
国家自然科学基金(编号:31260233) （编号:31260233）
