安徽医科大学学报Issue(5)：604-607,4.
LPS 对肝癌细胞株 HepG2增殖、凋亡及分泌炎症因子的影响
Effects of LPS on proliferation, apoptosis and secretion of cytokines of hepatocellular carcinoma cell line HepG2
摘要
Abstract
Objective To observe the effects of different concentrations of LPS on proliferation, apoptosis and se-cretion of cytokines of liver cancer cell line HepG2 in vitro, and to explore their possible mechanism. Methods The cells were divided into control group and 1, 10, 50, 100 μg / ml LPS treatment groups according to the method of random number. The proliferation of HepG2 cells at post treatment hour (PTH) 24, 48, 72 and 96 (denoted as absorption value) was detected by MTT and the apoptosis of HepG2 cells at PTH 24 was detected by flow cytome-ter. The level of IL-6 and IL-8 mRNA was detected by RT-PCR at PTH 24 and the concentration of IL-6 and IL-8 was detected at PTH 24 by chemiluminescence immunoassay. The data were analyzed with SPSS19. 0. Results Compared with the control group, proliferation of HepG2 cells was significantly increased (P < 0. 05) in the treat-ment groups at PTH 24 and 48, but there was no statistically significant difference in proliferation of HepG2 cells at PTH 72 and 96(P > 0. 05). There was no statistically significant difference in apoptosis rate of HepG2 cells at PTH 24 between the groups. Compared with the control group, the expression of IL-6 and IL-8 was significantly in-creased with the increase in concentration of LPS at PTH 24. Conclusion LPS can promote the proliferation of HepG2 cells in 48 hours and has no effect on apoptosis of HepG2 cells. LPS can also up-regulate the expression of IL-6 and IL-8 in HepG2 cells.关键词
脂多糖/HepG2 细胞/增殖/凋亡/炎症因子Key words
lipopolysaccharide/HepG2 cells/proliferation/apoptosis/inflammatory cytokines分类
医药卫生引用本文复制引用
刘亚婷,李涛,徐恩君,周敏,徐元宏,沈继龙..LPS 对肝癌细胞株 HepG2增殖、凋亡及分泌炎症因子的影响[J].安徽医科大学学报,2015,(5):604-607,4.基金项目
国家自然科学基金(编号:30801088、81201488) （编号:30801088、81201488）
卫生部应用研究项目“高通量 ELISA 检测系统化、标准化系列研究”(编号:28-1-50) （编号:28-1-50）
