安徽医科大学学报Issue(6)：782-786,5.
双酚 A 对小鼠腹腔巨噬细胞极化影响的体外研究
Effects of bisphenol A on polarization of mice peritoneal macrophage in vitro
摘要
Abstract
Objective To explore the effect of bisphenol A(BPA)on the polarization of mice peritoneal macro-phage in vitro. Methods Peritoneal macrophages isolated from C57BL/ 6J male mice were stimulated with murine (10 ng / ml)IFN-γ and LPS(500 ng / ml)for induction of M1-type macrophages,and(10 ng / ml)IL-4 for M2-type,respectively. Meanwhile cells were treated with 0. 1,1 and 10 μmol/ L BPA,blank control group and vehicle control group were established at the same time. The proportions of M1-type and M2-type macrophages were deter-mined by FACS analysis,the activities of iNOS(cytokines secreted by M1)and Arg-1(cytokines secreted by M2) were measured by ELISA assay. Results Different concentrations of BPA promoted M1-type polarization of mice peritoneal macrophage induced by IFN-γ and LPS. Compared with the blank control group,M1 type proportion was increased by 11. 3% and 17. 4% ,and iNOS activity was elevated by 1. 95 and 2. 29 times in 1 and 10 μmol/ L BPA group respectively,and the differences were statistically significant. Different concentrations of BPA inhibited M2-type polarization of mice peritoneal macrophage induced by IL-4. Compared with the blank control group,M2 type proportion was decreased by 9% and 14. 3% and Arg-1 activity was reduced by 0. 37 and 0. 49 times in 1 and 10 μmol/ L BPA group respectively,and the differences were statistically significant. Conclusion Low dose expo-sure of BPA can promote M1-type polarization of mice peritoneal macrophage,while inhibit M2-type polarization in vitro.关键词
双酚 A/巨噬细胞/极化Key words
bisphenol A/macrophages/polarization分类
医药卫生引用本文复制引用
李美玲,李应配,冷银芝,蒋建华,朱启星,沈彤..双酚 A 对小鼠腹腔巨噬细胞极化影响的体外研究[J].安徽医科大学学报,2015,(6):782-786,5.基金项目
国家自然科学基金 （）
