安徽医科大学学报Issue(6)：787-791,792,6.
苍耳亭对宫颈癌 SiHa 细胞增殖、凋亡和 GSTP1的影响
Effect of Xanthatin on proliferation,apoptosis and GSTP1 of cervical cancer SiHa cells
摘要
Abstract
Objective To investigate the effect of Xanthatin on proliferation,apoptosis and glutathione S-transferase P1(GSTP1)of cervical cancer SiHa cells. Methods MTS assay was used to study the effect of different concentra-tions of Xanthatin on the growth of SiHa cell lines. Morphological changes on SiHa cells were observed with fluores-cence microscope. The effect of Xanthatin on apoptosis of SiHa cells was analyzed by flow cytometry. In addition, changes of GSTP1 expression and activity were determined by immuohistochemistry and colorimetric method,and changes of p-JNK expression were determined by Western blot. Results Xanthatin(0 ~ 40 μmol/ L)significantly inhibited the proliferation on SiHa cells in a time and dose-dependent manner. The morphological changes observed in Xanthatin-treated cells included cell shrinkage,roundup and even some apoptotic bodies existed. After Xantha-tin treatment for 24 h in SiHa cells,the apoptotic ratios were 5. 02% ,9. 62% ,18. 5% ,26. 4% and 37. 5% with concentrations of Xanthatin at 0,5,10,20,40 μmol/ L. Compared with the control,apoptosis rate increased obvi-ously. SiHa cells exposed to increasing concentrations of Xanthatin led to the expression and activity of GSTP1 down-regulated and the expression of p-JNK. Conclusion These results demonstrate that Xanthatin exerts anti-tumor effects on SiHa cells through its anti-proliferative and pro-apoptotic roles. The molecular mechanism for pro-apoptotic effect may be related to its down-regulated effects on GSTP1 and up-regulated effects in c-Jun N-terminal kinase(JNK)pathway.关键词
苍耳亭/SiHa细胞/增殖/凋亡Key words
Xanthatin/SiHa cells/proliferation/apoptosis分类
医药卫生引用本文复制引用
任淑珍,史天陆,张蕾,姜玲,魏伟..苍耳亭对宫颈癌 SiHa 细胞增殖、凋亡和 GSTP1的影响[J].安徽医科大学学报,2015,(6):787-791,792,6.基金项目
安徽省自然科学基金（编号1408085MH198）；安徽高校省级自然科学研究 （）
