安徽医科大学学报Issue(7)：908-911,4.
microRNA-1271靶向调控白血病细胞CYLD蛋白的表达
microRNA-1271 regulates the expression of CYLD in leukemia cells
摘要
Abstract
Objective To explore the regulatory effect of microRNA-1271 on the expression of its target gene CYLD in leukemia cells. Methods microRNA-1271 expression levels in normal peripheral blood mononuclear cells( PB-MC) and leukemia cells were determined by quantitative real-time PCR. microRNA-1271 targeting CYLD 3′-UTR was predicted by TargetScan. 3′-UTR of CYLD was inserted into the dual luciferase reporter vector psi-CHECK2. The reporter activity was evaluated by the dual Luciferase Reporter Assay System after the luciferase promoter vector and microRNA were co-transferred into the HEK293A cell line. K562 cell lines were transfected with microRNA-1271 inhibitors ( anti-microRNA-1271 ) or a negative control microRNA ( anti-microRNA-NC ) , the expression of CYLD protein in the above transfected K562 cells were determined by Western blot. Results microRNA-1271 was up-regulated in human leukemia cell lines and primary leukemia cells compared to normal human PBMCs. The re-sults of dual luciferase assays validate CYLD as a specific target gene of microRNA-1271 . Inhibition of microRNA-1271 resulted in the upregulation of CYLD protein expression in K562 cell line. Conclusion microRNA-1271 is overexpressed in leukemia cells, the microRNA-1271 abnormal overexpression may play a key role in leukemia due to the down-regulation of CYLD.关键词
白血病/microRNA/microRNA-1271/CYLDKey words
leukemia/microRNA/microRNA-1271/CYLD分类
医药卫生引用本文复制引用
张茜,王媛媛,刘丽萍,倪芳..microRNA-1271靶向调控白血病细胞CYLD蛋白的表达[J].安徽医科大学学报,2015,(7):908-911,4.基金项目
国家自然科学基金青年项目(编号:31300715) （编号:31300715）
安徽省自然科学基金青年项目(编号:1308085QH136) （编号:1308085QH136）
