安徽医科大学学报Issue(7)：912-915,916,5.
二乙基亚硝胺诱导的肝癌小鼠TGF-βⅢ型受体的表达
The expression of type Ⅲ TGF-βreceptor in diethylnitrosamine-induced liver cancer model
摘要
Abstract
Objective To detect the expression of typeⅢTGF-β receptor( TβRⅢ) in diethylnitrosamine ( DEN)-induced liver cancer model. Methods C57BL/6J mice were intraperitoneally injected with DEN in a 20μg/g dos-age to establish liver cancer model. The pathological change of mice liver was observed by HE staining. The TGF- <br> β1 level in liver tissue was measured by ELISA assay. The expressions of TβRⅢ, p-Smad2 and Smad2 were detec-ted by Western blot. Results The mice liver cell of the control group formed normal size with the complete hepatic lobule structure. With the time passing, hepatic lobule in model group mice was destroyed gradually with liver cell dysplasia, pathology of fission, tumor nodule formation. In DEN-induced liver cancer model, the TGF-β1 level was elevated compared with the control group and the TβRⅢ protein expression was significantly decreased. The ex-pression of p-Smad2 protein was elevated, while Smad2 protein expression showed no obvious change. Conclusion The low expression of TβRⅢ may play an essential role in the progression of hepatocellular carcinoma.关键词
TβRⅢ/TGF-β1/肝细胞癌/二乙基亚硝胺Key words
TβRⅢ/TGF-β1/hepatocellular carcinoma/DEN分类
医药卫生引用本文复制引用
张森,孙妩弋,谷元婧,魏伟..二乙基亚硝胺诱导的肝癌小鼠TGF-βⅢ型受体的表达[J].安徽医科大学学报,2015,(7):912-915,916,5.基金项目
国家自然科学基金(编号:81300332,81330081) （编号:81300332,81330081）
高等学校博士学科点专项科研基金(编号:20113420120002) （编号:20113420120002）
安徽高校省级自然科学研究项目(编号:KJ2012A153) （编号:KJ2012A153）
