安徽医科大学学报Issue(7)：946-949,4.
TLR4 mAb对人正常肝内胆管上皮细胞LPS-TLR4-NF-κB通路的影响
Effect of TLR4 mAb on the normal intrahepatic of bile duct epithelial cells of human LPS-TLR4-NF-κB pathway
摘要
Abstract
Objective To observe the expression of Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB) in-duced by lipopolysaccharide ( LPS) and Toll-like receptor 4 monoclonal antibodies ( TLR4 mAb) in normal human intrahepatic biliary epithelial cells ( HiBEC) in vitro. Methods We cultured HiBEC in vitro. The cells were stim-ulated by LPS and different concentrations of TLR4 mAb. The levels of mRNA expression of TLR4 and NF-κB were detected by reverse transcription PCR ( RT-PCR) . Results LPS increased the mRNA expression of TLR4 and NF-κB ( P<0. 05 ) . TLR4 mAb reduced the mRNA expression of TLR4 and NF-κB ( P<0. 05 ) after LPS-stimulated HiBEC. High concentrations of TLR4 mAb reduced the mRNA expression of NF-κB (P<0. 05) after LPS-stimula-ted HiBEC in vitro. Conclusion The mRNA expression of TLR4 and NF-κB is increased afer LPS-stimulated Hi-BEC. TLR4 mAb antagonize the role of LPS on TLR4 and NF-κB,and reduce the mRNA expression of TLR4 and NF-κB.关键词
脂多糖/Toll 样受体4单克隆抗体/Toll样受体4/核转录因子-κBKey words
lipopolysaccharide/Toll-like receptor 4 monoclonal antibodies/Toll-like receptor 4/nuclear factor-κB分类
医药卫生引用本文复制引用
刘辉,余宏铸,韩珑,王正林..TLR4 mAb对人正常肝内胆管上皮细胞LPS-TLR4-NF-κB通路的影响[J].安徽医科大学学报,2015,(7):946-949,4.基金项目
2013年安徽省年度重点科研计划项目(编号:1301043034) （编号:1301043034）
