安徽医科大学学报Issue(8)：1058-1062,1063,6.
Ghrelin 预处理对 H9C2心肌细胞缺氧/复氧损伤的影响
Effect of pretreatment with Ghrelin on hypoxia/reoxygenation injury in H9C2 cells
摘要
Abstract
Objective To determine the effect of pretreatment with Ghrelin on hypoxia / reoxygenation (H/ R) inju-ry in rat cardiomyocytes. Methods Cultured H9C2 cells were randomly divided into 4 groups, including control group , H / R group , H / R + Ghrelin group , and H / R + Ghrelin + growth secretagogue receptor (GHSR)-siRNA group. H/ R injury was established by 21 hours of hypoxia and subsequent 6 hours of reoxygenation. Expression of growth GHSR in H9C2 cells was reduced by siRNA. Apoptosis of H9C2 cardiomyocytes under different treatments following H/ R injury was observed by Hoechst 33258 staining and apoptotic rate was detected by flow-cytometry a-nalysis. Besides, Western blot analysis was performed to detect the expression of the apoptosis related proteins in each group. Results GHSR was expressed in H9C2 cells and the expression of GHSR could be significantly knocked down by GHSR-siRNA. Compared with H/ R group, in H/ R + Ghrelin group the myocardium apoptotic rate was markedly decreased (P < 0. 05), the Bcl-2 / Bax ratio was increased (P < 0. 05) and the expression of caspase-3 protein was decreased (P < 0. 05). However, compared with H/ R + Ghrelin group, the apoptotic rate was increased(P < 0. 05), the Bcl-2 / Bax ratio was decreased (P < 0. 05) and the expression of caspase-3 protein was up-regulated (P < 0. 05) in H/ R + Ghrelin + GHSR-siRNA group. Conclusion Ghrelin provides concrete pro-tection against H/ R injury by inhibiting apoptosis in cardiomyocytes.关键词
H9C2 细胞株/缺氧/复氧损伤/Ghrelin/凋亡Key words
H9C2 cells/hypoxia/reoxygenation injury/Ghrelin/apoptosis分类
医药卫生引用本文复制引用
陈莹莹,吴继雄,王靓,何非..Ghrelin 预处理对 H9C2心肌细胞缺氧/复氧损伤的影响[J].安徽医科大学学报,2015,(8):1058-1062,1063,6.基金项目
国家自然科学基金(编号:81202631) （编号:81202631）
