安徽医科大学学报Issue(8)：1068-1071,4.
胶质瘤组织和细胞系中 Nanog 基因启动子区甲基化检测
Analysis and significance of Nanog promoter methylation status in gliomas and glioma cells
摘要
Abstract
Objective To detect the methylation status of Nanog gene promoter and the expression of Nanog mRNA in gliomas and glioma cell lines, and to explore the function of Nanog in glioma. Methods MSP and RT-PCR were conducted to detect Nanog gene promoter′s methylation status and its mRNA expression in normal brain tissues, gli-oma tissues, paracancerous tissues, glioma cell lines U87 and U251. Results Nanog gene promoter was hyperm-ethylated in gliomas, and the presence was significantly higher in gliomas than that in the paracancerous tissues (58. 82% vs 13. 63% )(χ2 = 14. 852,P < 0. 01). Nanog gene promoter unmethylation state was higher in grade Ⅲand grade Ⅳ glioma compared with grade I and grade Ⅱ tumors. Nanog gene promoter was hypermethylated in U87 and U251 cell lines. Nanog mRNA expression levels in high-grade(Ⅲ + Ⅳ) gliomas were higher than the low-grade(I + Ⅱ) group, Nanog mRNA expression was significantly increased in U87 and U251 cell lines. Conclusion Unmethylation state of Nanog gene promoter regions is one of the most important mechanisms that up-regulate its protein expression and influence the occurrence and development of gliomas.关键词
胶质瘤/Nanog/DNA 甲基化Key words
glioma/Nanog/DNA methylation分类
医药卫生引用本文复制引用
汪炎,牛朝诗,李仲颖,杨洋,贺虎,程传东,李静..胶质瘤组织和细胞系中 Nanog 基因启动子区甲基化检测[J].安徽医科大学学报,2015,(8):1068-1071,4.基金项目
国家自然科学基金(编号:81172407) （编号:81172407）
安徽省重点实验室绩效考核项目(编号:1306c083028) （编号:1306c083028）
