安徽医科大学学报Issue(8)：1090-1094,5.
miRNA-34a 对人胃癌 SGC-7901细胞增殖、凋亡的影响
Effects of miRNA-34a on the proliferation and apoptosis of gastric cancer SGC-7901 cells
摘要
Abstract
Objective To investigate the expression level of microRNA-34a (miRNA-34a,mir-34a) in human gas-tric cancer cell lines, and explore the role of mir-34a on the proliferation and apoptosis of human gastric cancer cell line SGC-7901. Methods The expression levels of mir-34a in human gastric mucosal epithelial cells GES and hu-man gastric cancer cell lines AGS, SGC-7901, MKN-45, BGC-823 were detected by RT-PCR. Gastric cancer SGC-7901 cells were infected with the lentiviral system expressing mir-34a in vitro. The infected cells status was e-valuated by fluorescence microscope. The proliferation, cell cycle and apoptosis were measured by MTT and flow cytometry. Results Compared with GES, the expression level of mir-34a decreased significantly in gastric cancer cell lines, especially in SGC-7901(P < 0. 01). The ratios of proliferation inhibition, cells arrested at G1 / G0 phase and apoptosis were significantly increased in mir-34a lentiviral infected group compared with the negative control group(P < 0. 05). Conclusion Mir-34a has lower expression level in some gastric cancer cell lines. Mir-34a can inhibit the proliferation, induce cell cycle arrest and cell apoptosis of gastric cancer cell line SGC7901.关键词
胃癌/SGC-7901 细胞/微小 RNA/microRNA-34a/增殖/凋亡Key words
gastric cancer/SGC-7901 cells/microRNA/microRNA-34a/proliferation/apoptosis分类
医药卫生引用本文复制引用
邓晓晶,邓敏,宋育林,王启之..miRNA-34a 对人胃癌 SGC-7901细胞增殖、凋亡的影响[J].安徽医科大学学报,2015,(8):1090-1094,5.基金项目
安徽省优秀青年人才基金重点项目(编号:2013SQRL053ZD) （编号:2013SQRL053ZD）
