安徽医科大学学报Issue(8)：1095-1098,1099,5.
长链非编码 RNA HOTAIR 对肾癌细胞增殖和凋亡的影响
Effect of long non-coding RNA HOTAIR on the proliferation and apoptosis of renal cancer cells
摘要
Abstract
Objective To investigate the effect of long non-coding RNA (LncRNA) Hox transcript antisense inter-genic RNA ( HOTAIR ) on the cell proliferation and apoptosis of renal cancer cell lines 786-O and ACHN. Methods Small interfere RNA ( siRNA ) that aims to down-regulate HOTAIR expression was transfected into two renal cell lines respectively, and the transfection efficiency was evaluated by qRT-PCR. Then the MTT assay,Ho-chest staining assay and enzyme-linked immunosorbnent assay(ELISA) were used to detect cell proliferation and apoptosis. Results The expression of HOTAIR could be down-regulated effectively by the siRNA (P < 0. 05). Down-regulation of HOTAIR could inhibit the proliferation(P < 0. 05) and increase apoptosis(P < 0. 05) of two re-nal cancer cells. Conclusion HOTAIR plays a role in promoting cell growth of renal cancer.关键词
肾癌/长链非编码 RNA/HOTAIR/细胞增殖/细胞凋亡Key words
renal cancer/long non-coding RNA/HOTAIR/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
夏明,黄卫人,蔡志明..长链非编码 RNA HOTAIR 对肾癌细胞增殖和凋亡的影响[J].安徽医科大学学报,2015,(8):1095-1098,1099,5.基金项目
国家重点基础研究发展计划(973计划)(编号:2014CB745200) （973计划）
国家自然科学基金(编号:81272840) （编号:81272840）
国家自然科学青年基金(编号:81201579) （编号:81201579）
