安徽医科大学学报Issue(8)：1102-1106,5.
氚照射对大鼠海马神经细胞黏附分子 L1、NCAM 表达的影响
Effects of irradiation from tritiated water on the expression of neural cell adhesion molecule L1 and NCAM of hippocampus in rats
摘要
Abstract
Objective To explore the effects of low concentrations of tritium irradiation using tritiated water on the expression of neural cell adhesion molecule L1 (L1) and neural cell adhesion molecule (NCAM) in vitro. Meth-ods Hippocampal neuron cells from neonatal Sprague-Dawley (SD) rats (24 h) were primarily cultured in DMEM for 7 d, followed by exposure to tritiated water at concentrations of 3. 7 × 102 , 3. 7 × 103 , 3. 7 × 104 , 3. 7 × 105 , 3. 7 × 106 Bq / ml for 24 h respectively, and cells without tritium irradiation served as control group (0 Bq / ml). Live Cell Imaging System was performed to detect neuronal migration capacity. Western blot and immunocytochemistry (ICC) were used to measure the expression levels of neural cell adhesion molecule L1 and NCAM. Results The migration distance of irradiated neural cells was significantly shorter than that of the control group and declined gradually with increasing tritiated water (HTO) concentrations (P < 0. 05); Western blot and ICC showed that the expression of L1 and NCAM were both presented a dose-dependent decrease in hippocampal neuron cells when irra-diated by tritium (P < 0. 05). Conclusion Neurons exposed to tritium irradiation are probably undergoing the de-creased expression of neural cell adhesion molecules, further leading to abnormal neuronal migration.关键词
大鼠/氚水/神经细胞黏附分子/神经细胞Key words
rats/tritiated water/neural cell adhesion molecule/neural cell分类
医药卫生引用本文复制引用
王永生,姚晓波,蔡二朋,邱俊,吴翠萍,王明明..氚照射对大鼠海马神经细胞黏附分子 L1、NCAM 表达的影响[J].安徽医科大学学报,2015,(8):1102-1106,5.基金项目
国家自然科学基金(编号:81273003) （编号:81273003）
安徽省自然科学基金(编号:1208085MH162) （编号:1208085MH162）
安徽省教育厅自然科学基金(编号:KJ2011Z162) （编号:KJ2011Z162）
