按摩与康复医学Issue(3)：122-124,3.
姜黄素+5-FU对粘液表皮样癌MEC-1细胞抑制作用的研究
Study on the Inhibitory Effect of Curcumin and 5-FU on MEC-1 Cells of Mucoepidermoid Carcinoma
摘要
Abstract
Objective:To investigate the inhibitory effect of curcumin and 5-FU on MEC-1 cells of mucoepidermoid carcinoma. Methods: MEC-1 cells were revived and cultured, cells reached a logarithmic growth phase were divided in experimental group, including curcumin group, 5-FU group, curcumin and 5-FU group, and control group which is PBS group; experimental group and control group were arranged concentration of 5μmol/L, 10μmol/L, 20μmol/L, 40μmol/L, 80μmol/L, 160μmol/L from low to high in the MTT experiment, to observe the effects on vitro growth ac-tivity of MEC-1 cells; in the flow cytometry analysis experiment, experimental group and control group were arranged moderate concentration of 20μmol/L, 40μmol/L, 80μmol/L, to observe the effects on cell cycle and apoptosis rate of tumor cells. Results:6 different concentration of experi-mental groups had obviously inhibitory effects on tumor cells and positive correlation with concentration and action time in MTT experiment;flow cytometry analysis showed that 3 different concentration of experimental groups had effects on cell cycle and apoptosis rate of MEC-1,and with the concentration increasing, G0/G1 phase decreased, S phase without significant changes, G2/M phase cells increased;apoptosis rate changes indicated that curcumin and 5-FU could induce the apoptosis of MEC-1 tumor cells more effectively than single group. Conclusion:Curcumin and 5-FU can ef-fectively inhibit the growth of mucoepidermoid carcinoma MEC-1 and make them apoptosis, and curcumin can enhance the inhibitory effect of 5-FU on MEC-1 cells.关键词
粘液表皮样癌/姜黄素/5-FU/MEC-1细胞/抑制Key words
mucoepidermoid cancer/curcumin/5-FU/MEC-1 cells/inhibition分类
医药卫生引用本文复制引用
朱雅文,吴发印,樊治英..姜黄素+5-FU对粘液表皮样癌MEC-1细胞抑制作用的研究[J].按摩与康复医学,2015,(3):122-124,3.基金项目
广东省珠海市卫生局项目资助，编号2013066 （）
