北华大学学报（自然科学版）Issue(4)：482-485,4.DOI:10.11713/j.issn.1009-4822.2014.04.014
肠球菌临床耐药性分析及耐药基因vanM在屎肠球菌中的检测
Analysis on Enterococcal Clinical Resistance and Detection of New Resistance Gene vanM in Enterococcus faecium
摘要
Abstract
Objective To investigate the antibiotic resistance of isolated Enterococcus spp. and the distribution of gene vanM in Enterococcus faecium. Methods VITEK60 automatic microorganism analyzer was used to conduct identification of Enterococci and drug sensitive test,and the results were calculated and analyzed according to the standard of NCCLS/CLSI;Glycopeptide drug resistance gene vanM was detected by PCR and the sequence of positive gene was measured for analyzing the relationship between the resistance gene and the resistance. Results 506 Enterococcus isolates, there were 277 isolates of Enterococcus faecium and 299 isolates of Enterococcus faecalis. The sample resources included urine 167(33. 0%),stool 114(22. 5%),sputum 78(15. 4%),bile 57 (11.3%),pus 54(10. 7%) and others 36(7. 1%) respectively. The drug sensitive test results showed that Enterococcus had a high antibiotic resistance to most antibiotics;the resistance rate of Enterococcus faecium toβ-lactams(ampicillin),quinolones(levofloxacin) and glycopeptide(vancomycin and teicoplanin) antibiotics was significantly higher than that of Enterococcus faecalis(P<0. 05);the detectable rate of vanM fragment was 100%and that of teicoplanin-resistant isolates was 90%. Conclusions Enterococcus faecium can cause all kinds of clinical infection and its resistant rate is significantly higher than that of Enterococcus faecalis,showing a multiple drug resistance trend. vanM gene may play an important role in the resistance mechanisms to glycopeptide antibiotics,vancomycin and teicoplanin.关键词
屎肠球菌/耐药/vanM基因Key words
Enterococcus faecium/resistance/vanM分类
医药卫生引用本文复制引用
刘晓薇,孙贺,李咏梅..肠球菌临床耐药性分析及耐药基因vanM在屎肠球菌中的检测[J].北华大学学报（自然科学版）,2014,(4):482-485,4.基金项目
吉林省科技发展计划项目(20110921) （20110921）
