北京生物医学工程Issue(2)：181-184,4.DOI:10.3969/j.issn.1002-3208.2015.02.12
基于双光子共聚焦成像的急性高眼压大鼠小梁网孔隙率变化研究
Change of porosity in trabecular meshwork under acute high intraocular pressure using two-photon microscope
摘要
Abstract
Objective To obtaln morphological change of trabecular meshwork under different pressure and do certaln basic research for exploring aqueous humor discharge. Methods Four SD rats were divided into A group and B group. Enucleated left eyes were perfused at pressure of 40 mm Hg(A group)and 60mmHg(B group)for 24 hours to achieve high IOP and right eyes were control group. We used two-photon confocal system to observe morphological images of trabecular meshwork in each eye:to split from fundus and expose the limbus,then we got the images each 2μm until fuzziness. Results The collagen fiber in trabecular meshwork of the control group arranged regularly. We could see obvious boundaries between trabecular meshwork and surrounding tissue. After perfused 40 mmHg pressure,the collagen fiber of trabecular meshwork became collapsed and merged with surrounding tissue. The fibers arranged in disorder. After perfused 60 mmHg,collagen fiber of trabecular meshwork fractured more obviously,juxtacanalicular connective tissue collapsed completely,and difficult to be distinguished from the surrounding tissue. Conclusions Acute intraocular pressure might cause the structure abnormal in aqueous humor discharge channel:trabecular meshwork tissue in anterior chamber became compressed,and sclera vein collapsed. This abnormal anatomy caused excreted difficulty of aqueous humor,and aggravated the elevated intraocular pressure in return.关键词
小梁网/高眼压/孔隙率/巩膜静脉/双光子共聚焦成像系统Key words
trabecular meshwork/high intraocular pressure/porosity/sclera vein/two-photon microscopy分类
医药卫生引用本文复制引用
任琳,梅曦,刘志成..基于双光子共聚焦成像的急性高眼压大鼠小梁网孔隙率变化研究[J].北京生物医学工程,2015,(2):181-184,4.基金项目
国家自然科学基金（31070840）、北京市自然科学基金（3122010）、首都医科大学重点实验室开放课题项目（2014LCSW01）资助 （）
