北京大学学报（医学版）Issue(2)：305-311,7.DOI:10.3969/j.issn.1671-167X.2015.02.023
人胚胎干细胞分化为角质形成细胞过程中标志物的表达特点
Expression characteristics of epithelial markers in human embryonic stem cells differentiating into keratinocytes
摘要
Abstract
Objective:To differentiate human embryonic stem cells ( hESCs ) into keratinocytes ( K-hESCs) and analyse the expression characteristics of biomarkers of K-hESCs.Methods: The hESCs of line H9 were seeded on matrigel in mTeSR1 medium.The hESCs were directly differentiated into kerati-nocytes in epithelial differentiation medium with bone morphogenetic protein 4, retinoic acid and N2 sup-plement.The karyotype of K-hESCs was analyzed, comparing the gene expression differences of K-hESCs with human gingival epithelial cells (HGECs), human immortalized oral epithelial cells (HIOECs) and HaCaT by Real-time PCR.Molecular characteristics of the cell differentiation were observed throughout the process by immunocytochemical techniques.Results:H9-hESCs were successfully differentiated into the cells that exhibited characteristics of keratinocytes in epithelial differentiation medium.The karyotype of K-hESCs was 46, XX; and the keratinocyte gene p63 expression in K-hESCs was significantly lower than that in HaCaT ( P<0.05) , but there was no significant difference of p63 expression in K-hESCs, comparing with that in HGECs and HIOECs ( P >0.05 ) .Conclusion: H9-hESCs could be directly differentiated into K-hESCs.The gene expression of K-hESCs was similar to that of epithelial cells in the early stage of monolayer cells differentiation with high proliferative activity.关键词
胚胎干细胞/角蛋白细胞/核型分析/上皮细胞Key words
Embryonic stem cells/Keratinocytes/Karyotyping/Epithelial cells分类
医药卫生引用本文复制引用
任玉兰,战园,路璐,李盛林,傅歆,俞光岩,曹彤,刘鹤..人胚胎干细胞分化为角质形成细胞过程中标志物的表达特点[J].北京大学学报（医学版）,2015,(2):305-311,7.基金项目
国家国际科技合作专项基金（2011DFA32190）资助 Supported by Foundation of International Technical Cooperation Projects （）
