北京中医药大学学报Issue(4)：241-246,6.DOI:10.3969/j.issn.1006-2157.2015.04.006
基于药物体系的紫苏子特征图谱质量表征关联分析研究∗
Quality representation and correlation analysis of Fructus Perillae based on characteristic spectrum of medicinal system
摘要
Abstract
Objective To establish the quality representation and correlation analysis method based on the characteristic spectrum of medicinal system of Fructus Perillae ( Zisuzi) , so as to evaluate quality of Zisuzi pieces effectively and accurately. Methods Using established HPLC-PDA method to characterize the quality of Zisuzi characteristic spectrum of medicinal system. The quality of 15 batches of Zisuzi pieces was represented according to the number and chemical type of characteristic peaks on characteristic spectrum, and the quantity of 15 batches of Zisuzi pieces was represented based on the content of three characteristic index components of luteolin, apigenin,rosemary acid, flavonoids ( represented by luteolin) and phenolic acids ( represented by rosemary acid). Correlation analysis of quality and quantity represen-tations was then performed referring to the benchmark pieces. Results As the reference substance, characteristic spectrum of Lot. 15 contains 12 characteristic peaks, 5 peaks of flavonoids and 7 peaks of phenolic acids, which all appeared on the chromatograms of 15 batches Zisuzi pieces. Lot. 15,6,9,10,2, 5 ,7 Zisuzi pieces had more effective index components. After comprehensive evaluation of more total ef-fective index components of Lot. 6 , 12 , 11 , 7 , 4 , 14 ,10 and high correlation degree of Lot. 6 , 12 , 11 , 7, 4, 14, 10 correlated with Lot. 15, the quality of Lot. 15, 6, 7, 10, 2, 5, 9, 12, 11,14 were judged to be better. Conclusion The established HPLC method by quality and quantity representation of characteristic spectrum of 15 batches of Zisuzi pieces is simple and accurate. And the evaluation mode of quantity and quality and their correlation based on Zisuzi medicinal system, concerning on systemic corre-lation and application validity, can evaluate the quality of Zisuzi pieces effectively and accurately. <br> characteristic index components of luteolin, apigenin,rosemary acid, flavonoids ( represented by luteolin) and phenolic acids ( represented by rosemary acid). Correlation analysis of quality and quantity represen-tations was then performed referring to the benchmark pieces. Results As the reference substance, characteristic spectrum of Lot. 15 contains 12 characteristic peaks, 5 peaks of flavonoids and 7 peaks of phenolic acids, which all appeared on the chromatograms of 15 batches Zisuzi pieces. Lot. 15,6,9,10,2, 5 ,7 Zisuzi pieces had more effective index components. After comprehensive evaluation of more total ef-fective index components of Lot. 6 , 12 , 11 , 7 , 4 , 14 ,10 and high correlation degree of Lot. 6 , 12 , 11 , 7, 4, 14, 10 correlated with Lot. 15, the quality of Lot. 15, 6, 7, 10, 2, 5, 9, 12, 11,14 were judged to be better. Conclusion The established HPLC method by quality and quantity representation of characteristic spectrum of 15 batches of Zisuzi pieces is simple and accurate. And the evaluation mode of quantity and quality and their correlation based on Zisuzi medicinal system, concerning on systemic corre-lation and application validity, can evaluate the quality of Zisuzi pieces effectively and accurately.关键词
紫苏子/特征图谱/质量评价/关联分析/木犀草素/芹菜素/迷迭香酸Key words
Fructus Perillae(Zisuzi)/characteristic spectrum/quality evaluation/correlation analysis/luteolin/apigenin/rosemary acid分类
医药卫生引用本文复制引用
唐雪阳,石任兵,安琪,孙道涵,温静,卢广英,许舒娅,黄亚婷,李钢,姜艳艳..基于药物体系的紫苏子特征图谱质量表征关联分析研究∗[J].北京中医药大学学报,2015,(4):241-246,6.基金项目
十二五国家科技支撑计划项目(No．2012BAI29B06),北京中医药大学创新团队资助项目(No．2011-CXTD-12) （No．2012BAI29B06）
