北京中医药大学学报Issue(5)：318-322,5.DOI:10.3969/j.issn.1006-2157.2015.05.007
三七总皂苷对高糖诱导大鼠视网膜微血管内皮细胞损伤的保护作用
Protective effects of total saponrins in Panax Notoginseng on high glucose-induced injury in cultured rat retinal capillary endothelial cells (RRCECs)
摘要
Abstract
Objective To observe the effects of total saponins of Panax Notoginseng ( PNS ) protection and expression of intercellular adhesion molecules-1 ( ICAM-1 ) in cultured rat retinal capillary endothelial cells ( RRCECs ) induced with high-concentration glucose.Methods Endothelial cells obtained from rat retinas were cultured in vitro, and the cells were identified using immunofluorescence. RRCECs were randomly divided into five groups: normal group, high glucose group, high glucose+20 mg/L PNS group, high glucose+50 mg/L PNS group, and high glucose+100 mg/L PNS group.Cell viability was measured with MTT method while cell number was detected with Trypan blue assay.The protein expression of ICAM-1 in RRCECs was measured by using Western blotting.Results According to MTT assay, the cell viability in high glucose+PNS (50 mg/L, 100 mg/L) groups were promoted after an action time of 48 hours and 72 hours (P<0.01).According to Trypan blue assay, after 48 hours and 72 hours, the cell number in high glucose+PNS (100 mg/L) group was increased ( P<0.01);after 48 hours, the cell number in high glucose+PNS (50 mg/L) group was increased (P<0.05).After an action time of 72 hours, the expression of ICAM-1 in all high glucose+PNS groups were detected by using Western blotting.The result showed that the expression of ICAM-1 in RRCECs decreased with the rise of PNS concentration under high glucose condition ( P <0.01 ) .Conclusion PNS could protect RRCECs from damage caused by high glucose, and inhibit the expression of ICAM-1.Relation between protective effects of PNS and regulation of ICAM-1 expression in cultured RRCECs merits further study.关键词
三七总皂苷/视网膜微血管内皮细胞/细胞间黏附分子-1/大鼠Key words
total Saponins of Panax Notoginseng/retinal capillary endothelial cells/intercellular adhesion molecules-1分类
医药卫生引用本文复制引用
乔园,段惠惠,黄建梅,唐民科..三七总皂苷对高糖诱导大鼠视网膜微血管内皮细胞损伤的保护作用[J].北京中医药大学学报,2015,(5):318-322,5.基金项目
“重大新药创制”国家科技重大专项（No．2011ZX09201－201－22），北京中医药大学研究生自主课题 （）
