北京中医药大学学报Issue(6)：388-392,5.DOI:10.3969/j.issn.1006-2157.2015.06.006
截断疗法与常规疗法对小鼠流感病毒性肺炎治疗作用的比较∗
Comparing truncated and routine methods in the treatment of mice pneumonia induced by influenza virus
摘要
Abstract
Objective To compare the effects of truncated and routine methods in the treatment of mice pneumonia induced by influenza virus. Methods ICR mice (n=80) were randomly divided into normal group, model group, routine treatment group and truncated treatment group. The last three groups were infected with mouse lung-adapted influenza virus strain by inoculating intranasally. One hour after the inoculation, the truncated treatment group was intragastrically given Xijiao dihuang Tang ( Rhinoceros Horn and Rehmannia Decoction) combined with Yinqiao San ( Lonicera and Forsythia Powder) decoction while the routine treatment group was intragastrically given Yinqiao San decoction only on the first three days and Xijiao dihuang Tang only on the following four days. All groups were treated for 7 consecutive days. The survival rate, average survival days and body weights were calculated during 14 days ’ observation. Balb/c mice ( n =128 ) were divided into the same four groups described above with the same corresponding intervention. These mice were sacrificed on the 2nd, 4th, 6th and 8th days respective-ly. Specimen were obtained and evaluated. The viral titers in lung homogenate and the lung index were <br> measured dynamically;the lung gross lesions and tissue pathological changes were observed on the 8 th day. Results The survival rate of truncated treatment group was twice as high as routine treatment group and average survival days also increased significantly; the lung index of truncated therapy group significantly decreased compared with the routine group on the 8th day. The viral titers of truncated treatment group in lung homogenate decreased on the 2 nd , 4 th , 6 th and 8 th day compared with the routine treatment group, yet the difference was not statistically significant. The pulmonary gross lesions and tissue pathological changes under light microscope were less severe than the routine treatment group. Conclusion Truncated method seemed to be superior to routine method in the treatment of severe influenza viral pneumonia mice. Its mechanisms may not lie in its stronger anti-viral effect, but in its stronger inhibitory effect on inflammatory cascade after viral infection compared with routine treatment.关键词
截断疗法/病毒性肺炎/犀角地黄汤合银翘散/流感病毒/小鼠Key words
truncated method/viral pneumonia/Xijiao dihuang Tang combined with Yinqiao San/influenza virus/mice分类
医药卫生引用本文复制引用
董莹莹,吴珺,陈丹军,李根茂,葛东宇,邱泽计,郝钰..截断疗法与常规疗法对小鼠流感病毒性肺炎治疗作用的比较∗[J].北京中医药大学学报,2015,(6):388-392,5.基金项目
国家自然科学基金资助项目(No.30772871),教育部博士点博导类基金资助项目(No.20130013110010),北京中医药大学自主选题资助项目(No.2014-JYBZZ-XS-020) （No.30772871）
