波谱学杂志Issue(2)：318-328,11.DOI:10.11938/cjmr20150214
Lin28特异性结合let-7 RNA结构基础
Structural Basis for Lin28 Specific Interaction with let-7 RNA
摘要
Abstract
The let-7 miRNA (microRNA) family control many cell-fate determination genes to influence pluripotency, differentiation, and transformation. Lin28 is a specific, posttranscriptional inhibitor of let-7 biogenesis. The C-terminal Zn-knuckle domain (ZKD) of Lin28 specially interacts with a conserved GGAG or GGAG-like motif in let-7 miRNA. We here report the NMR structure of human Lin28 binding to let-7 RNA with a sequence of 5′-A–2A–1G1G2A3G4-3′, demonstrating that the two folded domains of Lin28 ZKD recognize the region G1G2A3G4 of the RNA. All bases in bound RNA adopt anti conformation, and the backbone of RNA is bent due to Lin28 binding, consistent with the observations in the previous crystal structure, but different from those in the reported NMR structure, further confirming the structural basis for how Lin28 specially recognizes this RNA.关键词
let-7/Lin28/Zn-knuckle/核磁共振/结构Key words
let-7/Lin28/Zn-knuckle/NMR/structure分类
数理科学引用本文复制引用
卢秀秀,顾嘉琦,蓝文贤,王春喜,麻锦彪,曹春阳..Lin28特异性结合let-7 RNA结构基础[J].波谱学杂志,2015,(2):318-328,11.基金项目
Grants from the Ministry of Science and Technology of China (2011CB966300), the National Natural Science Foundation of China (21272261,21472229 and 21275154), and the Science and Technology Commission of Shanghai Municipality (15ZR1449300) （2011CB966300）
