吉林大学学报（医学版）Issue(3)：488-492,5.DOI:10.13481/j.1671-587x.20140305
FOXC2调控DLL4／Notch1信号通路对乳腺癌MCF-7细胞血管生成的影响
Influence of FOXC2 in angiogenesis of breast cancer MCF-7 cells by DLL4/Notch1 signal pathway
摘要
Abstract
Objective To explore the influence of tranSCription factor FOXC2 in angiogenesis of breast cancer MCF-7 cells and to clarify the action mechanism of FOXC2 in promoting tumor angiogenesis.Methods FOXC2 gene and empty vector gene were transfected into breast cancer of MCF-7 cell line with FOXC2 lentivirus gene transfection technique to obtain stable transfection cell line. The MCF-7 cells were devided into non-transfected group,empty-vector group and over-expression group.Matrigel assay and Transwell chamber test were used to observe the changes of tube formation and migration ability of human umbilical vein endothelial cells (HUVECs)in MCF-7 cells supernatant in various groups. PT-PCR and Western blotting methods were applied to detect the expressions of FOXC2,DLL4 and Notch1 mRNA and protein.Results Compared with non-tranfected group and empty-vector group,the tube formation and the migration number of HUVECs in FOXC2 over-expression group were increased(P<0.05);the expressions of FOXC2,DLL4 and Notch1 mRNA and proteins were significantly increased(P<0.05).Conclusion The FOXC2 over-expression in MCF-7 cells can increase the tube formation ability and migration ability of HUVECs,and its mechanism may be related to Notch signaling pathway.关键词
乳腺肿瘤/MCF-7 细胞/叉头框-C2/Notch信号通路/血管生成Key words
breast neoplasms/MCF-7 cells/forkhead box C-2/Notch signaling pathway/angiogenesis分类
医药卫生引用本文复制引用
刘红,谢佳,刘毫,郑曰勇,吴诚义,屈洪波,李聪..FOXC2调控DLL4／Notch1信号通路对乳腺癌MCF-7细胞血管生成的影响[J].吉林大学学报（医学版）,2014,(3):488-492,5.基金项目
国家自然科学基金资助课题 （）
